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612161 Anti-Tissue Factor Mouse mAb (TF9-10H10)

This Anti-Tissue Factor Mouse mAb (TF9-10H10) is validated for use in Dot Blot, ELISA, FC, Frozen Sections, WB, IF, Paraffin Sections, Neutralization Studies for the detection of Tissue Factor.

Anti-Tissue Factor Mouse mAb (TF9-10H10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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612161
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, PmMMonoclonal Antibody

Pricing & Availability

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612161-500UG
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      		 Plastic ampoule 500 μg
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      Description
      OverviewRecognizes the tissue factor protein.
      Catalogue Number612161
      Brand Family Calbiochem®
      Application Data
      Detection of human tissue factor by immunoblotting. Sample: Whole cell lysate from JEG-3 cells. Primary antibody: Anti-Tissue Factor Mouse mAb (TF9-10H10) (Cat. No. 612161) (1:1000). Detection: chemiluminescence.
      References
      Product Information
      FormLyophilized
      FormulationLyophilized from 100 mM mannitol, 150 mM PBS, pH 7.4.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesFlow Cytometry Veltrop, M.H., et al. 2001. Infect. Immun. 69, 2797. ELISA, Frozen Sections, Paraffin Sections, Immunocytochemistry Contrino, J., et al. 1994. Am. J. Pathol. 145, 1315. Original Clone, Immunoblotting, Dot Blot, Inhibition Studies, Neutralization Studies Morrissey, J.H., et al. 1988. Thromb. Res. 52, 247. Immunoblotting Ott, I., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 20, 874.
      Key Applications Dot Blot
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Not Neutralization Studies
      Paraffin Sections
      Application NotesDot Blot (see application references)
      ELISA (see application references)
      Flow Cytometry (see application references)
      Frozen Sections (see application references)
      Immunoblotting (1:1000, see application references)
      Immunofluorescence (see application references)
      Paraffin Sections (see application references)
      Neutralization Studies (not recommended)
      Application CommentsSpecific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified apoprotein of human tissue factor
      ImmunogenHuman
      Epitopewithin extracellular domain
      CloneTF9-10H10
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Primate
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      612161-500UG 04055977187748

      Documentation

      Anti-Tissue Factor Mouse mAb (TF9-10H10) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tissue Factor Mouse mAb (TF9-10H10) Certificates of Analysis

      TitleLot Number
      612161
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-January-2016 JSW
      ApplicationDot Blot (see application references)
      ELISA (see application references)
      Flow Cytometry (see application references)
      Frozen Sections (see application references)
      Immunoblotting (1:1000, see application references)
      Immunofluorescence (see application references)
      Paraffin Sections (see application references)
      Neutralization Studies (not recommended)
      Application Data
      Detection of human tissue factor by immunoblotting. Sample: Whole cell lysate from JEG-3 cells. Primary antibody: Anti-Tissue Factor Mouse mAb (TF9-10H10) (Cat. No. 612161) (1:1000). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the tissue factor protein.
      BackgroundTissue factor (TF) is the membrane receptor for coagulation factors VII and VIIa and is a cell-surface initiator of coagulation. It is the major molecule of this type and is critical for controlling hemostasis, thrombosis, and inflammation. TF is not generally present in or on cells within the vasculature, but following vascular injury, blood can come into contact with TF expressed by extravascular cells, resulting in activation of the coagulation cascade.
      HostMouse
      Immunogen speciesHuman
      Immunogenpurified apoprotein of human tissue factor
      Epitopewithin extracellular domain
      CloneTF9-10H10
      IsotypeIgG₁
      Specieshuman, primate, not rabbit
      FormLyophilized
      FormulationLyophilized from 100 mM mannitol, 150 mM PBS, pH 7.4.
      PreservativeNone
      CommentsSpecific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      Application referencesFlow Cytometry Veltrop, M.H., et al. 2001. Infect. Immun. 69, 2797. ELISA, Frozen Sections, Paraffin Sections, Immunocytochemistry Contrino, J., et al. 1994. Am. J. Pathol. 145, 1315. Original Clone, Immunoblotting, Dot Blot, Inhibition Studies, Neutralization Studies Morrissey, J.H., et al. 1988. Thromb. Res. 52, 247. Immunoblotting Ott, I., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 20, 874.