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ABE861 Anti-TRAF3

Anti-TRAF3 Antibody, Cat. No. ABE861, is a highly specific rabbit polyclonal antibody that targets TNF receptor-associated factor 3 (TRAF3) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-TRAF3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: TRAF3, CAP1, CRAF1 UniProt Number: Q13114
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ABE861
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE861
      ReplacesABE585
      DescriptionAnti-TRAF3
      Alternate Names
      • TNF receptor-associated factor 3
      • CAP-1
      • CD40 receptor-associated factor 1
      • CD40-binding protein
      • CD40BP
      • CRAF1
      • LAP1
      • LMP1-associated protein 1
      Background InformationTNF receptor-associated factor 3 (UniProt Q13114; also known as CAP-1, CD40 receptor-associated factor 1, CD40-binding protein, CD40BP, CRAF1, LAP1, LMP1-associated protein 1) is encoded by the TRAF3 (also known as CAP1, CRAF1) gene (Gene ID 7187) in human. NF-κB-inducing kinase (NIK) is negatively regulated by a complex composed of the E3 ubiquitin ligases c-IAP1 and 2 (cellular inhibitor of apoptosis 1 and 2) and the adaptors TRAF2 and 3 (TNF receptor-associated factors 2 and 3). The TRAF2-TRAF3 complex bridges the c-IAPs and NIK into close proximity, where TRAF2 recruits c-IAP1/2 and TRAF3 binds to NIK. NF-κB signaling activation results in TRAF3 degradation, leading to upregulated NIK. TRAF3 deletion in B-cells results in uncontrolled growth and TRAF3 mutations have been identified in several human B-cell cancers, including Hodgkin lymphoma, splenic marginal zone lymphoma, and multiple myeloma. TRAF3 also plays an indirect role in promoting PI3K/AKT and JAK/STAT signalings, which are negatively regulated by NIK. TRAF3 prevents NIK from targeting PTEN to the plasma membrane and thereby promoting PI3K/AKT signaling. TRAF3 is required for normal T cells to function properly and anaplastic large cell lymphoma (ALCL) cells reply on PI3K/AKT and JAK/STAT signaling for proliferation. Opposite to B-cell cancers, TRAF3 knockdown triggers cell cycle arrest in ALCL cells. Human TRAF3 contains one RING-type (a.a. 68-77) and two TRAF-type (a.a. 135-190 and 191-249) zinc fingers, a , coiled coil (a.a. 267-338) regin, followed a C-terminal MATH (meprin and TRAF homology) domain (a.a. 415-560) that mediates interaction with receptor cytomplasmic domain.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TRAF3 Antibody, Cat. No. ABE861, is a highly specific rabbit polyclonal antibody that targets TNF receptor-associated factor 3 (TRAF3) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TRAF3 in rat testis as well as in human kidney and pancreas tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence within the coiled coil region of human TRAF3
      Epitopedomain of:
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody targets a coiled coil region sequence conserved among bovine, equine, human, mouse, and rat TRAF3 protein sequences.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat. Predicted to react with Bovine, Horse, and Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TRAF3
      • CAP1
      • CRAF1
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~64 kDa observed. 64.49/55.31 kDa (human isoform 1/2), 64.29/64.19 kDa (mouse/rat) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected TRAF3 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE861 04054839077517