Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.
References
Product Information
Format
Culture Supernatant
Control
RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Detect Src using this Anti-Src Antibody, CT, clone NL19, validated for use in western blotting & immunoprecipitation.
Key Applications
Immunoprecipitation
Western Blotting
Application Notes
Western Blot Analysis: A 1:2000 to 1:8000 dilution of this lot detected Src in RIPA whole cell lysates from human A431 cells. Immunoprecipitation: 5-20 μL of a previous lot immunoprecipitated Src from 500 μg of A431 RIPA lysate.
Biological Information
Immunogen
KLH conjugated synthetic peptide (CKVADFGLARLIEDNEYTARQGA) of Src (avian sarcoma virus), residues 400-422. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
Clone
NL19
Host
Rabbit
Specificity
Src-tyrosine kinase family
Isotype
IgG
Species Reactivity
Human
Mouse
Rat
Species Reactivity Note
Predicted to cross react with frog and chicken. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
SIZE: 536 amino acids; 59835 Da SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight
~53-62kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
routinely evaluated by immunoblot on RIPA whole cell lysates from human A431 and Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
