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565942 Anti-Sialyl Lewis a Mouse mAb (KM231)

This Anti-Sialyl Lewis a Mouse mAb (KM231) is validated for use in Adhesion Blockade, ELISA, Flow Cytometry, Immunoblotting, Paraffin Sections Radioimmunoassay for the detection of Sialyl Lewis a.

Anti-Sialyl Lewis a Mouse mAb (KM231) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Sialyl Lea

565942
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the SLea carbohydrate epitope on glycoproteins and glycolipids on cell membranes and in the cytoplasm of breast, colon, ovarian and stomach cancers, and in lung adenocarcinoma.
      Catalogue Number565942
      Brand Family Calbiochem®
      SynonymsAnti-Sialyl Lea
      References
      ReferencesNagata, K., et al. 1994. J. Biol. Chem. 269, 23290.
      Sasaki, K., et al. 1993. J. Biol. Chem. 268, 22782.
      Product Information
      FormLiquid
      FormulationIn PBS, protein stabilizer, pH 7.2.
      Preservative≤0.1% sodium azide
      Applications
      Data setup Error. Pagelet <collection_feature_application_id-AB> not found.
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Radioimmunoassay
      Application NotesAdhesion Blockade (see comments)
      ELISA (see comments)
      Flow Cytometry (10 µg/ml)
      Immunoblotting (see comments)
      Paraffin Sections (5 µg/ml)
      Radioimmunoassay (see comments)
      Application CommentsReacts strongly with an antigen in cell membranes and cytoplasm of various cancerous tissues, including lung adeno-carcinomas, and cancers of the stomach, colon, breast, and ovary. Reacts weakly with various normal tissues, including stomach mucosa, bronchus, pancreatic acinar cells, germ cells, intestinal epithelial metaplasia, coated epithelium of the colon, and epithelia of the pancreatic duct, gall duct, and renal tubule. Does not react with fat cells, connective tissue, inflammatory cells, or red blood cells. The antibody has also been reported to work for immunoblotting, ELISA, RIA, and adhesion blocking. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman gastric cancer cells
      ImmunogenHuman
      CloneKM231
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      565942 0

      Documentation

      Anti-Sialyl Lewis a Mouse mAb (KM231) Certificates of Analysis

      TitleLot Number
      565942

      References

      Reference overview
      Nagata, K., et al. 1994. J. Biol. Chem. 269, 23290.
      Sasaki, K., et al. 1993. J. Biol. Chem. 268, 22782.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2007 RFH
      SynonymsAnti-Sialyl Lea
      ApplicationAdhesion Blockade (see comments)
      ELISA (see comments)
      Flow Cytometry (10 µg/ml)
      Immunoblotting (see comments)
      Paraffin Sections (5 µg/ml)
      Radioimmunoassay (see comments)
      DescriptionPurified mouse monoclonal antibody. Recognizes the Sialyl Lewis a epitope on glycoproteins ≥1000 kDa.
      BackgroundSialyl Lewis a [SLea; NeuAcα2,3Galβ1,3(Fucα1,4)GlcNAc] is a non-myeloid-specific tetrasaccharide that acts as a minimal ligand for E-selectin (ELAM; endothelial leukocyte adhesion molecule), an endothelial cell-surface glycoprotein that mediates recruitment of neutrophils and monocytes to sites of endothelial inflammation. This monoclonal antibody is specific for the SLea epitope found on various cell-surface glycoproteins and glycolipids.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman gastric cancer cells
      CloneKM231
      IsotypeIgG₁
      Specieshuman
      FormLiquid
      FormulationIn PBS, protein stabilizer, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsReacts strongly with an antigen in cell membranes and cytoplasm of various cancerous tissues, including lung adeno-carcinomas, and cancers of the stomach, colon, breast, and ovary. Reacts weakly with various normal tissues, including stomach mucosa, bronchus, pancreatic acinar cells, germ cells, intestinal epithelial metaplasia, coated epithelium of the colon, and epithelia of the pancreatic duct, gall duct, and renal tubule. Does not react with fat cells, connective tissue, inflammatory cells, or red blood cells. The antibody has also been reported to work for immunoblotting, ELISA, RIA, and adhesion blocking. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
      Toxicity Standard Handling
      ReferencesNagata, K., et al. 1994. J. Biol. Chem. 269, 23290.
      Sasaki, K., et al. 1993. J. Biol. Chem. 268, 22782.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies