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MABF2070-25UG Anti-Protein-tyrosine phosphatase eta (CD148) Antibody, clone 8A-1

Anti-Protein-tyrosine phosphatase eta (CD148), clone 8A-1, Cat. No. MABF2070, is a Syrian hamster monoclonal antibody that detects Receptor-type tyrosine-protein phosphatase eta (CD148) and is tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

Research Category: Inflammation & Immunology Gene Symbol: Ptprj, Byp, Scc1 UniProt Number: Q64455
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MABF2070-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, IH(P), WBSHmPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2070-25UG
      DescriptionAnti-Protein-tyrosine phosphatase eta (CD148) Antibody, clone 8A-1
      Alternate Names
      • Receptor-type tyrosine-protein phosphatase eta
      • EC: 3.1.3.48
      • Protein-tyrosine phosphatase eta
      • R-PTP-eta
      • HPTP beta-like tyrosine phosphatase
      • Protein-tyrosine phosphatase receptor type J
      • R-PTP-J
      • Susceptibility to colon cancer 1
      • CD148
      Background InformationReceptor-type tyrosine-protein phosphatase eta (UniProt: Q64455, also known as EC: 3.1.3.48, Protein-tyrosine phosphatase eta, R-PTP-eta, HPTP beta-like tyrosine phosphatase Protein-tyrosine phosphatase receptor type J, R-PTP-J, Susceptibility to colon cancer 1, CD148) is encoded by the Ptprj (also known as Byp, Scc1) gene (Gene ID: 19271) in murine species. R-PTP-eta is a monomeric, single pass type I membrane protein that is expressed at high levels in brain, kidney, spleen, and intestine; Lower expression levels are seen in liver, lung, thymus, and heart. It is also expressed at a high level in the myeloid cell line FDC-P2, and at a lower level in the pre-B lymphoid cell line WEHI-231. Fibroblast cell line NIH3T3 and the erythroid cell line F5-5 lack its expression. It is synthesized with a signal peptide (aa 1-28) that is subsequently cleaved off. The mature form has an extracellular domain (aa 29-876), a helical region (aa 877-897), a cytoplasmic domain (aa 898-1238). R-PTP-eta either dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2 and plays a role in cell adhesion, migration, proliferation and differentiation. It is also involved in vascular development and ma participate in the mechanism of contact inhibition of cell growth. It has a positive effect on cell-matrix adhesion and on platelet activation and thrombosis. It can also serve as a negative regulator of cell proliferation and has a negative effect on PDGF-stimulated cell migration via dephosphorylation of PDGFR. R-PTP-eta is also involved in T cell receptor (TCR) signaling where upon TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
      References
      Product Information
      FormatPurified
      PresentationPurified Syrian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Protein-tyrosine phosphatase eta (CD148), clone 8A-1, Cat. No. MABF2070, is a Syrian hamster monoclonal antibody that detects Receptor-type tyrosine-protein phosphatase eta (CD148) and is tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Protein-tyrosine phosphatase eta (CD148) in Western Blotting applications (Stepanek, O., et. al. (2011). J Biol Chem. 286(25):22101-12).

      Immunohistochemistry Analysis: A representative lot detected Protein-tyrosine phosphatase eta (CD148) in Immunohistochemistry applications (Katsumoto, T.R., et. al. (2013). J Clin Invest. 123(5):2037-48).

      Flow Cytometry Analysis: A representative lot detected Protein-tyrosine phosphatase eta (CD148) in Flow Cytometry applications (Stepanek, O., et. al. (2011). J Biol Chem. 286(25):22101-12; Lin, J., et. al. (2004). J Immunol. 173(4):2324-30).
      Biological Information
      Immunogen293T cells expressing murine CD148.
      Clone8A-1
      ConcentrationPlease refer to lot specific datasheet.
      HostSyrian Hamster
      SpecificityClone 8A-1 immunostains Protein-tyrosine phosphatase eta in  murine spleen tissue secctions.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Ptprj
      • Byp
      • Scc1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight136.77 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse spleen tissue.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody immunostained Protein-tyrosine phosphatase eta (CD148) in mouse spleen tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2070-25UG 04054839514821

      Documentation