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NA63 Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54)

This Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) is validated for use in Immunoblotting, IF, Paraffin Sections for the detection of Polypyrimidine Tract Binding Protein (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PTB

NA63
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes ~57 kDa PTB protein in Jurkat cells.
      Catalogue NumberNA63
      Brand Family Calbiochem®
      SynonymsAnti-PTB
      Application Data
      Detection of human polypyrimidine tract binding protein by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) (Cat. No. NA63) (1 μg/ml). Detection: chemiluminescence.
      References
      References<→EMD:if(output-is PDS)→> Gontarek, R. R., et al. 1999. Nucleic Acids Res. 27, 1457.
      Ito, T. and Lai, M. M. 1999. Virology 254, 288.
      Wagner, E. J., et al. 1999. Electrophoresis 20, 1082.
      Zhang, L, et al. 1999. RNA 5, 117.
      Huang, S., et al. 1997. J. Cell Biol. 137, 965.
      Valcarcel, J., and Gebauer, F. 1997. Curr. Biol. 7, R705.
      Brunel, F., et al. 1996. Nucleic Acids Res. 24, 1608. <→EMD:endif()→>
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate, 0.2% gelatin.
      Positive controlJurkat cells or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmunohistochemistry, Immunofluorescence, Original Clone Huang, S., et al. 1997. J. Cell Biol. 137, 965.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml)
      Immunofluorescence (1-2 µg/ml, see application references)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required see application references)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenHeLa cell nuclear extract
      ImmunogenHuman
      CloneSH54
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NA63 0

      Documentation

      Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) Certificates of Analysis

      TitleLot Number
      NA63

      References

      Reference overview
      <→EMD:if(output-is PDS)→> Gontarek, R. R., et al. 1999. Nucleic Acids Res. 27, 1457.
      Ito, T. and Lai, M. M. 1999. Virology 254, 288.
      Wagner, E. J., et al. 1999. Electrophoresis 20, 1082.
      Zhang, L, et al. 1999. RNA 5, 117.
      Huang, S., et al. 1997. J. Cell Biol. 137, 965.
      Valcarcel, J., and Gebauer, F. 1997. Curr. Biol. 7, R705.
      Brunel, F., et al. 1996. Nucleic Acids Res. 24, 1608. <→EMD:endif()→>
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      SynonymsAnti-PTB
      ApplicationImmunoblotting (1 µg/ml)
      Immunofluorescence (1-2 µg/ml, see application references)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required see application references)
      Application Data
      Detection of human polypyrimidine tract binding protein by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) (Cat. No. NA63) (1 μg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the ~57 kDa PTB protein.
      BackgroundPolypyrimidine tract binding protein (PTB) is an abundant vertebrate hnRNP protein that functions as a splicing regulator with multiple targets. PTB exists as a 57 kDa protein that is found exclusively in the perinucleolar compartment (PNC) and regulates alternative splicing by binding to a specific pyrimidine tract sequence that is typically found near the 3' end of introns. The PNC in turn is a structure that is more prevalent in cancer cells than in normal cells. This latter observation suggests the possibility of using expression of PTB as a marker for tumor cells. In solution phase, PTB exists as a homodimer and in some cancer cells exists as two isoforms, the ratio of which appears to correlate with metastatic potential particularly in prostatic carcinoma. PTB is involved in the regulation of many different viral genomes such as that of hepatitis C virus.
      HostMouse
      Immunogen speciesHuman
      ImmunogenHeLa cell nuclear extract
      CloneSH54
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlJurkat cells or breast carcinoma tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      References<→EMD:if(output-is PDS)→> Gontarek, R. R., et al. 1999. Nucleic Acids Res. 27, 1457.
      Ito, T. and Lai, M. M. 1999. Virology 254, 288.
      Wagner, E. J., et al. 1999. Electrophoresis 20, 1082.
      Zhang, L, et al. 1999. RNA 5, 117.
      Huang, S., et al. 1997. J. Cell Biol. 137, 965.
      Valcarcel, J., and Gebauer, F. 1997. Curr. Biol. 7, R705.
      Brunel, F., et al. 1996. Nucleic Acids Res. 24, 1608. <→EMD:endif()→>
      Application referencesImmunohistochemistry, Immunofluorescence, Original Clone Huang, S., et al. 1997. J. Cell Biol. 137, 965.