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MAB1281 Anti-Nuclei Antibody, clone 235-1

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MAB1281
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Human OnlyIH(P), IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1281
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nuclei Antibody, clone 235-1
      Alternate Names
      • HuNu
      Background InformationCell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models.
      Product Information
      FormatPurified
      Control
      • All human cell types
      PresentationHybridoma supernatant. Liquid in PBS. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect Nuclei using this Anti-Nuclei Antibody, clone 235-1 validated for use in IH(P) & IP.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot; use at 1:10-1:500.

      Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended.
      http://www.ihcworld.com/_protocols/antibody_protocols/nuclei_chemicon.htm

      Identifies an 82 and 70 kD protein by immunoprecipitation.

      Optimal working dilutions and protocols must be determined by the end user.
      Biological Information
      Clone235-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityStains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
      IsotypeIgG1
      Species Reactivity
      • Human Only
      Species Reactivity NoteThe human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      Stem Cell Type
      • Neural Stem Cells
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1281 04053252317378