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06-284-AF488 Anti-Nitrotyrosine Antibody AlexaFluor® 488 Conjugate

Anti-Nitrotyrosine AlexaFluor® 488 Conjugate, Cat. No. 06-284-AF488, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling
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06-284-AF488
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue Number06-284-AF488
      DescriptionAnti-Nitrotyrosine Antibody AlexaFluor® 488 Conjugate
      Alternate Names
      • Nitrotyrosine AlexaFluor® 488 Conjugate
      Background InformationNitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration. In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified Rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl,and 0.05% sodium azide, 10mg/ml BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nitrotyrosine AlexaFluor® 488 Conjugate, Cat. No. 06-284-AF488, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.
      Biological Information
      ImmunogenNitrated Keyhole Limpet hemocyanain (KLH).
      EpitopeNitrated Tyrosine
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects tyrosine nitrated proteins.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with all species.
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Purfied
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells treated with peroxytnitrite.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      06-284-AF488 04055977298840

      Documentation

      Anti-Nitrotyrosine Antibody AlexaFluor® 488 Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nitrotyrosine Antibody AlexaFluor® 488 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-Nitrotyrosine AlexaFluor® 488 -Q2610558 Q2610558
      Anti-Nitrotyrosine AlexaFluor® 488 Conjugate 2943137
      Anti-Nitrotyrosine AlexaFluor® 488 Conjugate 2902697
      Anti-Nitrotyrosine AlexaFluor® 488 Conjugate - 3314246 3314246
      Anti-Nitrotyrosine AlexaFluor® 488 Conjugate - 3524736 3524736
      Anti-Nitrotyrosine AlexaFluor® 488 Conjugate - 4096905 4096905
      Anti-Nitrotyrosine AlexaFluor® 488 Conjugate -2834502 2834502
      Anti-Nitrotyrosine AlexaFluor® 488 Conjugate Polyclonal Antibody 3091232