Millipore Sigma Vibrant Logo

482736 Anti-Nitric Oxide Synthase (2-12), Endothelial N-Terminal Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-eNOS

482736
  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~135 kDa myristoylated and non-myristoylated forms of eNOS.
      Catalogue Number482736
      Brand Family Calbiochem®
      SynonymsAnti-eNOS
      Product Information
      FormLyophilized
      FormulationLyophilized undiluted serum.
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1:800)
      Immunocytochemistry (1:400)
      Application CommentsDoes not cross-react with recombinant human inducible NOS (iNOS) or neuronal NOS (nNOS). Variables associated with assay conditions will dictate the proper working dilution.

      Recommended Procedure for Immunoblotting of Anti-eNOS:

      1. Run SDS-PAGE using 4-15% gradient gels or single percentage gels (e.g., 7.5%) and electrophoretically transfer to PVDF membrane.

      2. Block overnight with 4% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer.

      3. Wash 2X with TBS/Tween®-20 detergent.

      4. Apply the reconstituted rabbit anti-eNOS at a dilution of at least 1:500 in the following diluent: 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent buffer. Higher dilutions of the primary antibody may be required. Incubate 2-4 h.

      5. Wash 3X with TBS/Tween®-20 detergent.

      6. Apply HRP-conjugated, affinity-purified goat anti-rabbit IgG at the recommended dilution. Use 1% normal goat serum in evaporated goat milk diluted 1:5 with TBS/Tween®-20 detergent as the diluent. Incubate 1-2 h.

      7. Wash 3X with TBS/Tween®-20 detergent, then soak overnight in a reasonably large volume of the same buffer.

      8. Develop the blot using an HRP substrate suitable for immunoblotting, such as DAB.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 2-12 of human endothelial nitric oxide synthase (eNOS)
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 100 µl PBS, 1% BSA. Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solution.
      Global Trade Item Number
      Catalogue Number GTIN
      482736 0