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ABN90P Anti-NeuN purified Antibody

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ABN90P
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IH(P), ICCGpAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN90P
      DescriptionAnti-NeuN purified Antibody
      Alternate Names
      • NEUronal Nuclei
      • clone A60
      • NeuN
      Background InformationRNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. The NeuN is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. It is widely expressed in various regions of the brain, including the cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord. However, it is not detected in Cajal-Retzius cells in the neocortex, Purkinje cells, and photoreceptor cells. NeuN expression has been reported as early as embryonic day 9.5 (E9.5) and by E12.5 it can be found in the developing ventral horns and is also detected in the developing dorsal horns and in the dorsal root ganglion. It is expressed from embryonic stage to adulthood. Its expression is enhanced by retinoic acid treatment. It can serve as a pre-mRNA alternative splicing regulator and regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay. Anti-NeuN antibodies can identify most types of neurons in the whole nervous system with some rare exceptions. Antibodies for NeuN predominantly associate with cell nuclei and, to a lesser extent, with the perinuclear cytoplasm. Two isoforms of the NeuN protein (46 and 48 kDa) are present in both locations but differ in their relative concentration in the nucleus and cytoplasm.NeuN knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume. They show increased susceptibility to seizures and reduced anxiety-related behaviors compared with wild type littermates. (Ref.: Lin, YS et al. (2018). PLOS One. 13(2); e0192355; Mao, S., et al. (2016). Front. Neuroanat. 13;10:54).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse E16 brain tissue lysate
      PresentationPurified guinea pig polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NeuN purified, Cat. No. ABN90P, is a guinea pig polyclonal antibody that detects NeuN and is tested for use in Western Blotting and Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NeuN in mouse brain tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first 97 amino acids of mouse NeuN.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGuinea Pig
      SpecificityThis Guinea pig polyclonal antibody detects NeuN. It targets an epitope within the N-terminal region.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • NeuN
      Purification MethodAntigen Affinity Purified
      Molecular Weight~48 kDa observed; 40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in E16 Mouse brain tissue lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected NeuN in E16 Mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN90P 04053252647635

      Documentation

      Anti-NeuN purified Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-NeuN purified Antibody Certificates of Analysis

      TitleLot Number
      Anti-NeuN purified 3083062
      Anti-NeuN purified - 2123666 2123666
      Anti-NeuN purified - 2445032 2445032
      Anti-NeuN purified - 2069374 2069374
      Anti-NeuN purified - 2145116 2145116
      Anti-NeuN purified - 2201953 2201953
      Anti-NeuN purified - 2272164 2272164
      Anti-NeuN purified - 2329178 2329178
      Anti-NeuN purified - 3158068 3158068
      Anti-NeuN purified - 3226535 3226535

      References

      Reference overviewPub Med ID
      Diffusion tensor and volumetric magnetic resonance measures as biomarkers of brain damage in a small animal model of HIV.
      Lentz, MR; Peterson, KL; Ibrahim, WG; Lee, DE; Sarlls, J; Lizak, MJ; Maric, D; Reid, WC; Hammoud, DA
      PloS one  9  e105752  2014

      Show Abstract
      25144656 25144656

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies