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MABT63 Anti-Nectin-3/PVRL3 (CD113) Antibody, clone N3.12.4

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MABT63
50 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT63
      DescriptionAnti-Nectin-3/PVRL3 (CD113) Antibody, clone N3.12.4
      Alternate Names
      • poliovirus receptor-related 3
      • poliovirus receptor-related protein 3
      • nectin 3
      • nectin-3
      • Nectin-3
      • CD113 antigen
      Background InformationNectin-3, also known as Poliovirus receptor-related protein 3 (PVRL3) and PRR3, is thought to work with cadherin to form cell-cell adherens junctions. It is a transmembrane protein that is commonly found in the testis and in placental tissue. Nectin-3 colocalizes with CD155, the cognate receptor for poliovirus, to cell-cell junctions. Nectin-3's involvement in down-regulation PVR from the cell surface leads to a reduction in cell motility and proliferation.
      Product Information
      FormatPurified
      Control
      • U251 cells
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nectin-3/PVRL3 (CD113) Antibody, clone N3.12.4 detects level of Nectin-3/PVRL3 (CD113) & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenFull length recombinant protein corresponding to human Nectin-3.
      CloneN3.12.4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells. [supplied by OMIM].
      Gene Symbol
      • CDw113
      • PRR3
      • PPR3
      • CD113
      • PVRR3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.

      SUBUNIT STRUCTURE: Cis- and trans-homodimer. Can form trans-heterodimers with PVRL1/nectin-1, PVRL2/nectin-2, PVR, IGSF4B/Necl-1 and with IGSF4. Interacts with MLLT4/afadin. Binds with low affinity to TIGIT.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein Potential.

      TISSUE SPECIFICTY: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

      SEQUENCE SIMILARITIES: Belongs to the nectin family.

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

      SEQUENCE CAUTION: The sequence AAH17572.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight61 kDa calculated
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in U251 cells.

      Flow Cytometry Analysis: 0.1 µg of this antibody detected Nectin-3 in U251 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABT63 04053252414671