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MAB5778-I-100UL Anti-NMDAR2B Antibody, C-term Antibody, clone 1C6.5C4

Anti-NMDAR2B, C-term, clone 1C6.5C4, Cat. No. MAB5778-I, is a mouse monoclonal antibody that detects NMDAR2B and is tested for use in Western Blotting.

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MAB5778-I-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB5778-I-100UL
      DescriptionAnti-NMDAR2B Antibody, C-term Antibody, clone 1C6.5C4
      Alternate Names
      • Glutamate receptor ionotropic NMDA 2B
      • GluN2B
      • Glutamate [NMDA] receptor subunit epsilon-2
      • N-methyl D-aspartate receptor subtype 2B
      • NR2B
      Background InformationGlutamate receptor ionotropic, NMDA 2B (UniProt: Q00960; also known as GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B) is encoded by the Grin2b gene (Gene ID: 24410) in rat. NMDA receptor complexes are ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. They are involved in long-term potentiation and neuronal plasticity and regulate the number of nerve cells during development. However, over-stimulation NMDA receptors can lead to neuronal degeneration. NMDAR2B is a multi-pass membrane protein, which is synthesized with a signal peptide (aa 1-26), that is subsequently cleaved off to generate the mature form that contains two extracellular domains, three cytoplasmic domains, and four transmembrane domains. It is strongly expressed at birth and its levels increase until P6 to P20 and then begins to decline to the adult level. NMDAR2B is a component of the NMDA receptor complex that functions as heterotetrameric, ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. The channel activation requires binding of glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+. (Ref.: Laurie, DJ., et al. (1997). Mol. Brain Res. 51(1-2); 23-32).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-NMDAR2B, C-term, clone 1C6.5C4, Cat. No. MAB5778-I, is a mouse monoclonal antibody that detects NMDAR2B and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected NMDAR2B in Western Blotting applications (Laurie, D.J., et. al. (1997). Mol. Brain Res. 51(1-2):23-32).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 136 amino acids from the C-terminal half of Rat NMDAR2B.
      EpitopeC-terminal half
      Clone1C6.5C4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1C6.5C4 is a mouse monoclonal antibody that detects NMDAR2B. It targets an epitope within 136 amino acids from the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Rabbit
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rabbit, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Grin2b
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~170 kDa observed; 166.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected NMDAR2B in Rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5778-I-100UL 04065266553734