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MABN2397 Anti-MYCBP2 Antibody, clone PM-8A9

Anti-MYCBP2 Antibody, clone PM-8A9, Cat. No. MABN2397, is a highly specific mouse monoclonal antibody that targets MYCBP2 (PAM) and has been tested in Immunoprecipitation and Western blotting.

Anti-MYCBP2 Antibody, clone PM-8A9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: MYCBP2, KIAA0916, PAM UniProt Number: O75592
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MABN2397
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2397
      DescriptionAnti-MYCBP2 Antibody, clone PM-8A9
      Alternate Names
      • E3 ubiquitin-protein ligase MYCBP2
      • Myc-binding protein 2
      • Pam/highwire/rpm-1 protein
      • Protein associated with Myc
      Background InformationE3 ubiquitin-protein ligase MYCBP2 (UniProt: O75592; also known as Myc-binding protein 2, Pam/highwire/rpm-1 protein, Protein associated with Myc, MYCBP2) is encoded by the MYCBP2 (also known as KIAA0916, PAM) gene (Gene ID: 23077) in human.MYCBP2 protein mediates the ubiquitination and subsequent proteasomal degradation of TSC2/tuberin. It is ubiquitously expressed, but brain and thymus display exceptionally abundant expression. It is shown to co-localize with TSC1 and TSC2 along the neurites and in the growth cones. MYCBP2 may also function as a facilitator or regulator of transcriptional activation by MYC and have a role during synaptogenesis. It is reported to negatively regulate neuronal growth, synaptogenesis, and synaptic strength. Mice with MYCBP2-deficiency in peripheral sensory neurons show prolonged thermal hyperalgesia. Loss of MYCBP2 is also linked to constitutively activated p38 MAPK and increased expression of several proteins involved in receptor trafficking. Down-regulation of MYCBP2 in the spinal cord of adult rats is shown to enhance their. MYCBP2 is also shown to be an inhibitor of adenylyl cyclase. (Ref.: Holland, S et al. (2011). J. Biol. Chem. 286(5): 3671-3680).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MYCBP2 Antibody, clone PM-8A9, Cat. No. MABN2397, is a highly specific mouse monoclonal antibody that targets MYCBP2 (PAM) and has been tested in Immunoprecipitation and Western blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot detected MYCBP2 (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).

      Western Blotting Analysis: A representative lot detected endogenous PAM (MYCBP2)and overexpressed Myc-tagged PAM (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).
      Biological Information
      ImmunogenMBP (Maltose-binding protein)-tagged recombinat fragment of 150 amino acids from the N-terminal region of human MYCBP2.
      EpitopeN-terminus
      ClonePM-8A9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone PM-8A9 specifically detects MYCBP2 in HEK293 cells. It targets an epitope within the first 150 amino acids in the N-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYCBP2
      • KIAA0916
      • PAM
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~460 kDa observed; 510.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysates.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected MYCBP2 in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2397 04054839176449