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ABS44 Anti-MAPK 1/2 Antibody

Detect MAPK 3/MAPK 1 (ERK1/ERK2) using this Anti-MAPK 1/2 Antibody validated for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-MAPK 1/2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: Erk1, Erk2, Mapk, Mapk1, Mapk3, Prkm1, Prkm3 UniProt Number: P21708
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ABS44
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, HWB, IP, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS44
      Replaces06-182
      DescriptionAnti-MAPK 1/2 Antibody
      Alternate Names
      • ERK-1
      • ERT2
      • Extracellular signal-regulated kinase 1
      • Insulin-stimulated MAP2 kinase
      • MAP kinase 3
      • MAP kinase isoform p44
      • MAPK 3
      • Microtubule-associated protein 2 kinase
      • Mitogen-activated protein kinase 3
      • MNK1
      • p44-ERK1
      • p44-MAPK
      • ERK-2
      • ERT1
      • Extracellular signal-regulated kinase 2
      • MAP kinase 1
      • MAP kinase 2
      • MAP kinase isoform p42
      • MAPK 1
      • MAPK 2
      • Mitogen-activated protein kinase 1
      • Mitogen-activated protein kinase 2
      • p42-MAPK
      Background InformationMitogen-activated protein kinase 3 (EC 2.7.11.24; UniProt P21708; also known as ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, MNK1, p44-ERK1, p44-MAPK) is encoded by the Mapk3 (also known as Erk1, Prkm3) gene (Gene ID 50689) in rat species. ERK1 (a.k.a. MAPK 3) and ERK2 (a.k.a. MAPK 1 or MAPK 2) are related serine/threonine kinases of the Ras-Raf-MEK-ERK signaling pathway. ERK1/2 are activated by MEK1/2-catalyzed phosphorylation at their conserved Thr-Glu-Tyr (TEY) dual phosphorylation motif, first at the Tyr residue (human/rat ERK1 Tyr204/Tyr203, human/rat ERK2 Tyr187/Tyr183) and then at the Thr residue (human/rat ERK1 Thr202/Thr201, human/rat ERK2 Thr185/Thr181). ERK1/2 are proline-directed kinases that preferentially catalyze the phosphorylation of Pro-Xxx-Ser/Thr-Pro sequence motif in hundreds of cytoplasmic and nuclear substrates, including transcription factors such as Ets, Elk, and c-Fos. Besides this primary structure requirement, many ERK1/2 substrates possess a D-docking site and/or an F-docking site. A variety of scaffold proteins, including KSR1/2, IQGAP1, MP1, and β-Arrestin1/2, also participate in the regulation of ERK1/2 MAP kinase cascade. Ras-Raf-MEK-ERK signaling activity is upregulated in about one-third of all human cancers, and targeted inhibition against components of this signaling pathway represents a popular anticancer strategy.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • A431 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect MAPK 3/MAPK 1 (ERK1/ERK2) using this Anti-MAPK 1/2 Antibody validated for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected MAPK 3/MAPK 1 (ERK1/ERK2) in BALB/3T3, A431, and L6 cell lysates.



      Western Blotting (SNAP ID) Analysis: 1-2 µg/mL from a representative lot detected MAPK 3/MAPK 1 (ERK1/ERK2) in BALB/3T3, A431, and L6 cell lysates.



      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MAPK 3/MAPK 1 (ERK1/ERK2) in NIH/3T3, A431, HeLa, and C2C12 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal sequence of rat MAPK 3 (ERK-1).
      EpitopeC-terminal
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes MAPK 3/MAPK 1 (ERK1/ERK2) C-terminal sequence.
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Species Reactivity NoteReacts with human, mouse, and rat. Expected to react with bovine and canine based on 94% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Erk1
      • Erk2
      • Mapk
      • Mapk1
      • Mapk3
      • Prkm1
      • Prkm3
      Purification MethodProtein A purfied.
      UniProt Number
      Molecular Weight~44/42 kDa observed. 41.26/36.30 kDa (human MAPK1 isoform 1/2), 41.14 kDa (rat MAPK1), 41.14 kDa (mouse MAPK1), 43.00/38.14 kDa (human MAPK3 isoform 1/2), 42.95/45.64 kDa (rat MAPK3 isoform 1/2), 42.93 kDa (mouse MAPK3) calculated (Met1 removed). Target sequence is not present in human MAPK3 spliced isoform 3 (ERK1b).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.



      Western Blotting Analysis: 0.5 µg/mL of this antibody detected MAPK 3/MAPK 1 (ERK1/ERK2) in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS44 04053252324109

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