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MABS274 Anti-LRP6, clone A59, Ectodomain Antibody

This Anti-LRP6 antibody, clone A59, Ectodomain is validated for use in flow cytometry, IP & ICC for the detection of LRP6.

Anti-LRP6, clone A59, Ectodomain Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Insulin/Energy Signaling Gene Symbol: LRP6 UniProt Number: O75581
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MABS274
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS274
      DescriptionAnti-LRP6, clone A59, Ectodomain Antibody
      Alternate Names
      • Low-density lipoprotein receptor-related protein 6
      • LRP-6
      Background InformationLow density lipoprotein receptor-related protein 6, also known as LRP6, is a member of the low density Lipoprotein receptor gene family (LDLR). LDLR are cell surface proteins. LRP6 plays a crucial role in embryonic development by functioning as a co-receptor for the Wnt signaling pathway. LRP6 is highly homologous and presents itself as a dimer in the cell membrane until it is phosphlorylated by Wnt. LRP6 has a high affinity with the Dickkopf (DKK) family of antagonists which, when bound, block Wnt. When LRP6 binds to Wnt it is phosphlorated at multiple sites, which results in Axin, a scaffolding protein, being recruited to the cell membrane and β-catenin activation. β-catenin prevents Wnt and LRP6 from binding. LRP6 is associated with autosomal dominant coronary artery disease type 2. Mutations resulting in the omission of the extracellular domain of LPR6 results in constant activity whereas carboxy intracellular domain exclusion has a negative effect, resulting in the blocking of Wnt signaling.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-LRP6 antibody, clone A59, Ectodomain is validated for use in flow cytometry, IP & ICC for the detection of LRP6.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesFlow Cytometry Analysis: A representative lot from an independent laboratory detected LRP6, Ectodomain in Chimeric LRP6 expressing 293T cells (Yasui, N., et al. (2010). J Immunol Methods. 352(1-2):153-160.).

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated LRP6, Ectodomain from Wnt3a-treated HEK293T cell lysate (Yasui, N., et al. (2010). J Immunol Methods. 352(1-2):153-160.).

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected LRP6, Ectodomain in HeLa S3 cells (Yasui, N., et al. (2010). J Immunol Methods. 352(1-2):153-160.).
      Biological Information
      ImmunogenPurified protein corresponding to the ectodomain domain of human LRP6.
      EpitopeEctodomain Domain
      CloneA59
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LRP6
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight180 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HEK293 cells.

      Flow Cytometry Analysis: 2 µg of this antibody detected LRP6, Ectodomain in 1X10E6 HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS274 04053252955457

      Documentation

      Anti-LRP6, clone A59, Ectodomain Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-LRP6, clone A59, Ectodomain Antibody Certificates of Analysis

      TitleLot Number
      Anti-LRP6, clone A59, Ectodomain - Q2383199 Q2383199
      Anti-LRP6, clone A59, Ectodomain Monoclonal Antibody 3049399
      Anti-LRP6, clone A59, Ectodomain Monoclonal Antibody 2878134

      References

      Reference overviewPub Med ID
      Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation.
      Yasui, Norihisa, et al.
      J. Immunol. Methods, 352: 153-60 (2010)  2010

      Show Abstract       		19945460 19945460

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies