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ABN2290-100UL Anti-LHFPL5

Anti-LHFPL5, Cat. No. ABN2290, is a rabbit polyclonal antibody that detects LHFPL5 and is tested for use in Immunocytochemistry and Western Blotting.

ABN2290-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABN2290-100UL
      DescriptionAnti-LHFPL5
      Alternate Names
      • LHFPL tetraspan subfamily member 5 protein
      • Lipoma HMGIC fusion partner-like 5 protein
      • Tetraspan membrane protein of hair cell stereocilia
      Background InformationLHFPL tetraspan subfamily member 5 protein (UniProt: Q8TAF8; also known as Lipoma HMGIC fusion partner-like 5 protein, Tetraspan membrane protein of hair cell stereocilia) is encoded by the LHFPL5 (also known as TMHS) gene (Gene ID: 222662) in human. LHFPL5 is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. Its localization to the plasma membrane requires the presence of protocadherin related 15 (PCDH15). LHFPL5 is recognized as a critical component of the mechano-transduction complex in inner-ear hair cells. It is shown to physically interact with and stabilize transmembrane channel-like protein 1 (TMC1). The C-terminal half of LHFPL5 is involved in this interaction. The N-terminal half is involved in its dimerization and interaction with PCDH15. Mutations in LHFPL5 gene are known to cause autosomal recessive deafness that is characterized by non-syndromic sensorineural hearing loss from damage to the neural receptors of the inner ear. Knockout of LHFPL5 in murine models is shown to result in TMC1 reduction in the hair bundles of auditory cells. (Ref.: Yu, X., et al. (2020). Proc, Natl. Acad. Sci. USA. 117(47); 29894-29903).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in 0.02 mg/mL sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-LHFPL5, Cat. No. ABN2290, is a rabbit polyclonal antibody that detects LHFPL5 and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected LHFPL5 in Western Blotting applications (Yu, Z., et. al. (2020). Proc Natl Acad Sci USA. 117(47); 29894-29903).

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LHFPL5 in HEK293 cells transfected with LHFPL5 (TMHS) protein.

      Immunocytochemistry Analysis: A representative lot detected LHFPL5 in Immunocytochemistry applications (Yu, Z., et. al. (2020). Proc Natl Acad Sci USA. 117(47); 29894-29903).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human LHFPL tetraspan subfamily member 5 protein (LHFPL5).
      EpitopeC-terminal
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects LHFPL tetraspan subfamily member 5 protein (LHFPL5). It targets an epitope within 20 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LHFPL5
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight24.2 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LHFPL5 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2290-100UL 04065269802440