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MAB1794H Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated

Detect CD19 using this Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD19, B4, MGC12802, Leu-12 UniProt Number: P15391
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MAB1794H
100 assays  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMPhycoerythrinMonoclonal Antibody
      Description
      Catalogue NumberMAB1794H
      ReplacesCBL141P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated
      OverviewBIOCHEMISTRY:

      p I = 7.0
      Alternate Names
      • B-lymphocyte Surface Antigen B4
      Product Information
      FormatPhycoerythrin
      PresentationPurified from mouse ascitic fluid. R-Phycoerythrin conjugated. The antibody is supplied in 1.0ml PBS 0.01M, Sodium Azide 0.1%, pH 7.1, 0.2% BSA. The characteristics of each lot are tested by electrophoresis and flow cytometry.
      Quality LevelMQ100
      Applications
      ApplicationDetect CD19 using this Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated validated for use in FC.
      Key Applications
      • Flow Cytometry
      Application NotesThis antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry.

      SUGGESTED USAGE

      Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E(6) peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
      Biological Information
      ImmunogenRaji cells
      CloneFMC63
      HostMouse
      SpecificityThis specificity of this antibody is equivalent to that of CD19 monoclonal antibodies (Zola et al., 1988, 1989), based on comparative studies, antigen recognition and positive reaction with a CD19 transfected cell line. It detects a 96 kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. The antibody reacts with most B cell malignancies including chronic lymphocytic leukemia and most lymphomas, but not multiple myeloma.

      Cell reactivity:

      Normal:Stains all B lymphocytes in peripheral blood and spleen. Negative on granulocytes, monocytes, platelets, erythrocytes and T lymphocytes.

      Clinical:

      B cell chronic lymphocytic leukemia (B-CLL) +

      Pro lymphocytic leukemia (PLL +

      Hairy cell leukemia (HCL) +

      Common acute lymphoblastic leukemia (CALL) +

      Pre-B acute lymphoblastic leukemia (pre-B-ALL) +

      NULL-acute lymphoblastic leukemia (NULL-ALL) +

      T-acute lymphoblastic leukemia (T-ALL) -

      T-common lymphocytic leukemia (T-CLL -
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
      Gene Symbol
      • CD19
      • B4
      • MGC12802
      • Leu-12
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15391 # Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
      SIZE: 556 amino acids; 61128 Da
      SUBUNIT: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
      DISEASE:SwissProt: P15391 # Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265].
      SIMILARITY: SwissProt: P15391 ## Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.

      WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
      Packaging Information
      Material Size100 assays
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1794H 04053252579516