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382160 Anti-Histone H4, Acetylated (2-19) Rabbit pAb

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, TRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~10 kDa acetylated histone H4 protein in Tetrahymena and sodium butyrate-treated HeLa cells.
      Catalogue Number382160
      Brand Family Calbiochem®
      Application Data
      Detection of human acetylated histone H4 by immunoblotting. Samples: Acid-extracted proteins from HeLa cells treated with sodium butyrate at antibody concentrations of 0.5 µg/ml (lane 1) and 2 µg/ml (lane 2). Primary antibody: Anti-Histone H4, Acetylated (2-19) Rabbit pAb (Cat. No. 382160) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
      Braunstein, M., et al. 1993. Genes Devel. 7, 592.
      Perry, C.A., et al. 1993. Biochemistry 32, 13605.
      Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
      Positive controlTetrahymena and sodium butyrate-treated HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (0.5-2 µg/ml)
      Immunocytochemistry (5-10 µg/ml)
      Immunoprecipitation (5µg/500 µg lysate)
      Application CommentsThe antibody detected acetylated histone H4 in acid-extracted proteins from HeLa cells. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with histone H2B from HeLa cells. May cross-react with other acetylated proteins. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H4. For immunocytochemistry, human A431 cells were treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h and fixed with ice-cold 1% paraformaldehyde in PBS. Variables associated with assay conditions will dictate the optimal dilution.
      Biological Information
      Immunogena tetra-acetylated synthetic peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS(C)] corresponding to amino acids 2-19 of Tetrahymena histone H4, conjugated to KLH
      ImmunogenTetrahymena
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Tetrahymena
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      382160 0

      Documentation

      Anti-Histone H4, Acetylated (2-19) Rabbit pAb Certificates of Analysis

      TitleLot Number
      382160

      References

      Reference overview
      Braunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
      Braunstein, M., et al. 1993. Genes Devel. 7, 592.
      Perry, C.A., et al. 1993. Biochemistry 32, 13605.
      Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      ApplicationImmunoblotting (0.5-2 µg/ml)
      Immunocytochemistry (5-10 µg/ml)
      Immunoprecipitation (5µg/500 µg lysate)
      Application Data
      Detection of human acetylated histone H4 by immunoblotting. Samples: Acid-extracted proteins from HeLa cells treated with sodium butyrate at antibody concentrations of 0.5 µg/ml (lane 1) and 2 µg/ml (lane 2). Primary antibody: Anti-Histone H4, Acetylated (2-19) Rabbit pAb (Cat. No. 382160) (2 µg/ml). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~10 kDa acetylated histone H4 protein.
      BackgroundThe acetylation of core histones regulates gene expression through alterations in the chromatin structure. Lysine acetylation in the amino-terminal domains of histones H3 and H4 can alter the topology of the DNA in chromatin and probably influence its higher-order folding.
      HostRabbit
      Immunogen speciesTetrahymena
      Immunogena tetra-acetylated synthetic peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS(C)] corresponding to amino acids 2-19 of Tetrahymena histone H4, conjugated to KLH
      IsotypeIgG
      SpeciesTetrahymena, human
      Positive controlTetrahymena and sodium butyrate-treated HeLa cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe antibody detected acetylated histone H4 in acid-extracted proteins from HeLa cells. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with histone H2B from HeLa cells. May cross-react with other acetylated proteins. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H4. For immunocytochemistry, human A431 cells were treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h and fixed with ice-cold 1% paraformaldehyde in PBS. Variables associated with assay conditions will dictate the optimal dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
      Braunstein, M., et al. 1993. Genes Devel. 7, 592.
      Perry, C.A., et al. 1993. Biochemistry 32, 13605.
      Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.