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AB5808-200UL Anti-HCN4 Antibody

This Anti-HCN4 Antibody is validated for use in IH, WB for the detection of HCN4.

Anti-HCN4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: HCN4 UniProt Number: Q9Y3Q4
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AB5808-200UL
200 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5808-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-HCN4 Antibody
      Alternate Names
      • Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-HCN4 Antibody is validated for use in IH, WB for the detection of HCN4.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:200 using ECL on rat brain membranes.

      Immunohistochemistry using rat brain sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGST fusion protein corresponding to amino acids 119-155 of human HCN4 (Accession XP_007730, NP_005468, CAB42604, CAB52754).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4). The epitope does not share homology with any other known proteins.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HCN4
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9Y3Q4 # Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli.
      SIZE: 1203 amino acids; 129042 Da
      SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus.
      DOMAIN:SwissProt: Q9Y3Q4 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
      DISEASE: SwissProt: Q9Y3Q4 # Defects in HCN4 are a cause of autosomal dominant sick sinus syndrome (SSS) [MIM:163800]; also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors, in which case it is considered to be a congenital disorder.
      SIMILARITY: Belongs to the potassium channel HCN family. & Contains 1 cyclic nucleotide-binding domain.
      MISCELLANEOUS: Inhibited by extracellular cesium ions.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB5808-200UL 04053252507878

      Documentation

      Anti-HCN4 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-HCN4 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2453133 2453133
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) - 3276120 3276120
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2938948
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 3074963
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2907669
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 3106566
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 3305202
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2001220 2001220
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2042715 2042715
      RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2064273 2064273
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      References

      Reference overviewApplicationSpeciesPub Med ID
      Targeted insertion of the Cre-recombinase gene at the phenylethanolamine n-methyltransferase locus: a new model for studying the developmental distribution of adrenergic cells
      Ebert, Steven N, et al.
      Dev Dyn, 231:849-858 (2004)  2004

      		15517585 15517585
      Postnatal development of the hyperpolarization-activated excitatory current Ih in mouse hippocampal pyramidal neurons
      Vasilyev, Dmitry V and Barish, Michael E
      J. Neurosci., 22:8992-9004 (2002)  2002

      		ImmunofluorescenceMouse12388606 12388606