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PC154 Anti-Excitatory Amino Acid Transporter (GLT-1) Rabbit pAb

Anti-Excitatory Amino Acid Transporter (GLT-1), rabbit polyclonal recognizes the ~75 kDa and the ~50 kDa forms of GLT-1. It is validated for use in Western blotting and immunoprecipitation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC154
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
RRbPolyclonal Antibody

Pricing & Availability

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PC154-100UL
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      		 Plastic ampoule 100 ul
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      Description
      OverviewRecognizes the ~75 kDa and the ~50 kDa forms of GLT-1.
      Catalogue NumberPC154
      Brand Family Calbiochem®
      References
      ReferencesArriza, J.L., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 4155.
      Nagao, S., et al. 1997. Neuroscience 78, 929.
      Rothstein, J.D., et al. 1995. Ann. Neurol. 14, 5559.
      Arriza, J.L., et al. 1994. J. Neurosci. 14, 5559.
      Rothstein, J.D., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 6591.
      Kanai, Y., et al. 1992. Nature 360, 467.
      Pines, G., et al. 1992. Nature 360, 464.
      Rothstein, J.D., et al. 1992. N. Engl J. Med. 326, 1464.
      Storck T., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 10955.
      Product Information
      FormLiquid
      FormulationSerum diluted in PBS.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (see comments)
      Application CommentsThis antibody may also cross react nonspecifically with a ~102 kDa protein not believed to be related to GLT-1. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (NGKSADCSVEEEPWKREK) corresponding to amino acids at the C-terminus of rat GLT-1
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC154-100UL 04055977209488

      Documentation

      Anti-Excitatory Amino Acid Transporter (GLT-1) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Excitatory Amino Acid Transporter (GLT-1) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC154

      References

      Reference overview
      Arriza, J.L., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 4155.
      Nagao, S., et al. 1997. Neuroscience 78, 929.
      Rothstein, J.D., et al. 1995. Ann. Neurol. 14, 5559.
      Arriza, J.L., et al. 1994. J. Neurosci. 14, 5559.
      Rothstein, J.D., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 6591.
      Kanai, Y., et al. 1992. Nature 360, 467.
      Pines, G., et al. 1992. Nature 360, 464.
      Rothstein, J.D., et al. 1992. N. Engl J. Med. 326, 1464.
      Storck T., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 10955.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-October-2007 RFH
      ApplicationImmunoblotting (1:1000)
      Immunoprecipitation (see comments)
      DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes the ~75 kDa and the ~50 kDa forms of GLT-1.
      BackgroundPrecise regulation of excitatory amino acids (EAA) such as glutamate is essential, as glutamate is not only the major excitatory neurotransmitter, but also a potent excitotoxin. Defects in functional glutamate transport have been identified in the amyotrophic lateral sclerosis (ALS) brain and spinal cord, suggesting that defective glutamate transport may contribute to motor neuron degeneration. Three glutamate transporter subtypes have been identified in mammals, which include GLAST, GLT-1 and EAAC1. More recently, the homologous proteins EAAT1, EAAT2, and EAAT3 have been characterized in humans, respectively. EAAT4 and EAAT5 transporters have been localized to Purkinje cell synapses, and retina, respectively. The selective loss of glutamate transport in ALS appears to be specific for the GLT-1 (EAAT2) transporter subtyp. While GLT-1, GLAST and EAAC1 share approximately 50% sequence homology, the sequence used as the immunogen is unique to GLT-1.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide (NGKSADCSVEEEPWKREK) corresponding to amino acids at the C-terminus of rat GLT-1
      IsotypeIgG
      Speciesrat
      FormLiquid
      FormulationSerum diluted in PBS.
      Preservative≤0.1% sodium azide
      CommentsThis antibody may also cross react nonspecifically with a ~102 kDa protein not believed to be related to GLT-1. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesArriza, J.L., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 4155.
      Nagao, S., et al. 1997. Neuroscience 78, 929.
      Rothstein, J.D., et al. 1995. Ann. Neurol. 14, 5559.
      Arriza, J.L., et al. 1994. J. Neurosci. 14, 5559.
      Rothstein, J.D., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 6591.
      Kanai, Y., et al. 1992. Nature 360, 467.
      Pines, G., et al. 1992. Nature 360, 464.
      Rothstein, J.D., et al. 1992. N. Engl J. Med. 326, 1464.
      Storck T., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 10955.