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ABE76-I-25UG Anti-Enterobacterio Phage MS2 Coat Protein

Anti-EnterobacterioPhage MS2 Coat Protein, Cat. No. ABE76-I, is a highly specific rabbit polyclonal antibody that targets Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein and has been tested in Immunoprecipitation and Western Blotting.

Anti-Enterobacterio Phage MS2 Coat Protein MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function UniProt Number: P03612
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ABE76-I-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VirusIP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE76-I-25UG
      DescriptionAnti-Enterobacterio Phage MS2 Coat Protein
      Alternate Names
      • Capsid protein
      • CP
      • Coat protein
      • MS2 Coat Protein
      • Enterobacterio Phage MS2 Coat Protein
      Background InformationThe bacteriophage MS2 is a small, male-specific RNA bacteriophage that infects E. coli and other members of the Enterobacteriaceae family. Its genome comprises a single sense (+) strand of RNA 3569 nucleotides in length, which encodes only four proteins: the coat protein, the A or maturation protein, which is required for infectivity, a subunit of the viral replicase, and a small lysis protein encoded in an overlapping reading frame. The capsid protein (UniProt: P03612; also known as CP, Coat protein) of bacteriophage MS2 is a translational repressor. It is reported to inhibit the synthesis of the viral replicase by binding a specific RNA structure that contains the replicase translation initiation region. Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry and consists of 89 capsid proteins dimers (178 capsid proteins). It is involved in viral genome encapsidation through the interaction between a capsid protein homodimer and the multiple packaging signals present in the RNA genome. Proline 78 is shown to be essential for viral infectivity. (Ref.: Hill, HR et al. (1997). J. Mol. Biol. 266(1); 1-7).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-EnterobacterioPhage MS2 Coat Protein, Cat. No. ABE76-I, is a highly specific rabbit polyclonal antibody that targets Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein and has been tested in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:5,000 dilution from a representative lot detected recombinant EnterobacterioPhage MS2 Coat Protein and wildtype MS2 protein (Courtesy of Caroline Horizny from CNSE at SUNY Albany, Albany, NY).

      Immunoprecipitation Analysis: A 1:10,000 dilution from a representative lot detected EnterobacterioPhage MS2 Coat Protein in K562 cells treated with Escherichia phage MS2 (Bacteriophage MS2).
      Biological Information
      ImmunogenHis-tagged recombinant Enterobacteriophage MS2 Coat Protein (aa 2-130).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects MS2 bacteriophage capsid protein. The immunogen used has an N- terminus His tag and mutations to avoid dimerization and capsid formation.
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus.
      Antibody TypePolyclonal Antibody
      Purification MethodAffinity Purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 treated with Escherichia phage MS2 (Bacteriophage MS2) and K562+MS2 recombinant protein treated with Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected EnterobacterioPhage MS2 Coat Protein in K562 ells treated with Escherichia phage MS2 (Bacteriophage MS2) and K562+MS2 recombinant protein treated with Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE76-I-25UG 04054839437984

      Documentation

      Anti-Enterobacterio Phage MS2 Coat Protein SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Enterobacterio Phage MS2 Coat Protein Certificates of Analysis

      TitleLot Number
      Anti-Enterobacterio Phage MS2 Coat Protein - 3490047 3490047
      Anti-Enterobacterio Phage MS2 Coat Protein - 3695850 3695850
      Anti-Enterobacterio Phage MS2 Coat Protein - 3977218 3977218
      Anti-Enterobacterio Phage MS2 Coat Protein - 4058999 4058999
      Anti-Enterobacterio Phage MS2 Coat Protein - 4084405 4084405
      Anti-Enterobacterio Phage MS2 Coat Protein - 4128241 4128241