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MABF3126-25UG Anti-DR4/CD261 Antibody, clone DJR1

Anti-DR4/CD261, clone DJR1, Cat. No. MABF3126, is a mouse monoclonal antibody that detects DR4/CD261 and is tested for use in Flow Cytometry.

MABF3126-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3126-25UG
      DescriptionAnti-DR4/CD261 Antibody, clone DJR1
      Alternate Names
      • Tumor necrosis factor receptor superfamily member 10A
      • Death receptor 4
      • TNF-related apoptosis-inducing ligand receptor 1
      • TRAIL receptor 1
      • TRAIL-R1
      Background InformationTumor necrosis factor receptor superfamily member 10A (UniProt: O00220; also known as Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1, TRAIL receptor 1, TRAIL-R1, CD261) is encoded by the TNFRSF10A (also known as APO2, DR4, TRAILR1) gene (Gene ID: 8797) in human. CD261/DR4 is a single-pass type I membrane protein, which is synthesized with a signal peptide (aa 1-23) that is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-239), a transmembrane domain (aa 240-262), and a cytoplasmic domain (aa 263-468). It is widely expressed with high levels observed in spleen, peripheral blood leukocytes, small intestine, and thymus. TRAIL receptors (DR4 and DR5) induce apoptosis via caspase activation. The adapter molecule FADD is reported to recruit caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation that initiates the subsequent cascade of caspases mediating apoptosis. O-GlcNAcylation of DR4 is reported to be crucial for TRAIL death signaling. Both TRAIL-sensitive and TRAIL-resistant breast cancer cell lines can be efficiently killed by overexpression of the CD261/DR4. However, the extent of cell death depends on the strength of the promoter driving DR4 expression. (Ref.: Lee, H., et al. (2019). Cancer Res. 79; 2839-2852; Kazhdan, I., and Marciniak, RA (2004). Cancer Gene Ther. 11(10); 691-698).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-DR4/CD261, clone DJR1, Cat. No. MABF3126, is a mouse monoclonal antibody that detects DR4/CD261 and is tested for use in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Application NotesTested applications

      Flow Cytometry: A representative lot detected DR4/CD261 in Flow Cytometry applications (Uno, K., et al. (2003). Blood. 101(9); 3658-67; Lee, H., et al. (2019). Cancer Res. 79(11); 2839-2852).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenRecombinant fragment corresponding to the extracellular domain of human DR4/CD261 Fc fusion protein.
      EpitopeExtracellular domain
      CloneDJR1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone DJR1 is a mouse monoclonal antibody that detects DR4/CD261. It targets an epitope within the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TNFRSF10A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight50.09 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in BHK cells transfected with Human DR4.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected DR4/CD261 in BHK cells transfected with Human DR4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3126-25UG 04065269802518