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CC12 Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bcl-1, Anti-PRAD-1

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CC12
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

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CC12-100UG
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      Description
      OverviewRecognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
      Catalogue NumberCC12
      Brand Family Calbiochem®
      SynonymsAnti-Bcl-1, Anti-PRAD-1
      References
      ReferencesInaba, T., et al. 1992. Genomics 13, 565.
      Jiang, W., et al. 1992. Cancer Res. 2, 2980.
      Pines, J. 1992. Cell Growth Differ. 2, 305.
      Xiong, Y., et al. 1992. Genomics 13, 575.
      Xiong, Y., et al. 1992. Cell 71, 504.
      Motokura, T., et al. 1991. Nature 350, 512.
      Solomon, M.J., et al. 1990. Cell 63, 1013.
      Draetta, G., and Beach, D. 1988. Cell 54, 17.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controltrp E (Ab-1)
      Positive controlMCF7, or A431 cells or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Immunoprecipitation, and Neutralization Studies Lukas, J., et al. 1994. Oncogene 9, 707. Immunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Lukas, J., et al. 1994. Oncogene 9, 707. Paraffin and Frozen Sections Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Gillett, C., et al. 1994. Cancer Res. 54, 1812-1817.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Neutralization Studies
      Paraffin Sections
      Application NotesFlow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)
      Frozen Sections (see application references)
      Immunoblotting (1 µg/ml)
      Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
      Immunoprecipitation (see application references)
      Neutralization Studies (see application references)
      Paraffin Sections (5 µg/ml, heat pre-treatment required)
      Application CommentsDoes not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human cyclin D1
      ImmunogenHuman
      CloneDCS-6
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      CC12-100UG 04055977228465