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MABF1976 Anti-Complement Receptor C5aR Antibody, clone 20/70

Anti-Complement Receptor C5aR, clone 20/70, Cat. No. MABF1976, is a rat monoclonal antibody that detects Complement 5a receptor (C5aR) and has been tested for use in Flow Cytometry, Activity Assay, Inhibition of Activity/Function, Immunocytochemistry, and Neutralizing.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: C5ar1, C5ar, C5r1 UniProt Number: P30993
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MABF1976
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MINHIB, ACT, FC, ICC, NEUTRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF1976
      DescriptionAnti-Complement Receptor C5aR Antibody, clone 20/70
      Alternate Names
      • C5a anaphylatoxin chemotactic receptor 1
      • C5a anaphylatoxin chemotactic receptor
      • C5a-R
      • C5aR
      • CD88
      Background InformationC5a anaphylatoxin chemotactic receptor 1 (UniProt: P30993; also known as C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88) is encoded by the C5ar1 (also known as C5ar, C5r1) gene (Gene ID: 12273) in murine species. C5aR is a multi-pass membrane protein of the G-protein coupled receptor 1 family that serves as a receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. C5aR is a homodimer, but can also form higher-order oligomers. C5aR becomes phosphorylated on serine residues in response to C5a binding that results in internalization of the receptor and short-term desensitization to C5a. The phosphorylated receptor is known to Interact with arrestin beta 1 and 2 (ARRB1 and 2) and this interaction is associated with internalization of C5aR. Sulfation is also reported to play a critical role in the association of C5aR with C5a, but it does not alter the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Complement Receptor C5aR, clone 20/70, Cat. No. MABF1976, is a rat monoclonal antibody that detects Complement 5a receptor (C5aR) and has been tested for use in Flow Cytometry, Activity Assay, Inhibition of Activity/Function, Immunocytochemistry, and Neutralizing.
      Key Applications
      • Inhibits Activity/Function
      • Activity Assay
      • Flow Cytometry
      • Immunocytochemistry
      • Neutralizing
      Application NotesNeutralizing Analysis: A representative lot neutralized Complement receptor C5aR (Godau, J., et. al. (2004). J Immunol. 173(5):3437-45).

      Immunocytochemistry Analysis: A representative lot detected Complement receptor C5aR in Immunocytochemistry applications (Soruri, A., et. al. (2003). Immunol Lett. 88(1):47-52).

      Inhibits Activity/Function Analysis: A representative lot inhibited Complement receptor C5aR in Activity/Function applications (Godau, J., et. al. (2004). J Immunol. 173(5):3437-45; Soruri, A., et. al. (2003).

      Flow Cytometry Analysis: A representative lot detected Complement receptor C5aR in Flow Cytometry applications (Shushakova, N., et. al. (2002). J Clin Invest. 110(12):1823-30).

      Activity Assay Analysis: A representative lot detected Complement receptor C5aR in Activity Assay applications (Shushakova, N., et. al. (2002). J Clin Invest. 110(12):1823-30).
      Biological Information
      ImmunogenRat basophilic leukemia RBL-2H3 cells stably expressing murine C5aR.
      Epitopeextracellular domain
      Clone20/70
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 20/70 detects complement 5a receptor in murine species. It targets an epitope with in the extracellular domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • C5ar1
      • C5ar
      • C5r1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight39.02 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in BALB/c mouse bone marrow cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected Complement receptor C5aR in 1 million BALB/c mouse bone marrow cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF1976 04054839323331