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PC25 Anti-Cdc2/Cdk1 (Ab-1) Rabbit pAb

Anti-Cdc2/Cdk1 (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cdk1, Anti-p34

PC25
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~34 kDa Cdc2/Cdk1 protein in proliferating HeLa cells.
      Catalogue NumberPC25
      Brand Family Calbiochem®
      SynonymsAnti-Cdk1, Anti-p34
      Application Data
      References
      ReferencesReed, S.I., and Wittenberg, C. 1990. Proc. Natl. Acad. Sci. USA 87, 5697.
      Draetta, G., et al. 1987. Cell 50, 319.
      Simanis, V., and Nurse, P. 1986. Cell 45, 261.
      Reed, S.I., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 4055.
      Piggott, J.R., et al. 1982. Nature 298, 391.
      Nurse, P., and Bisset, Y. 1981. Nature 292, 558.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlProliferating human or rodent cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Application CommentsImmunoprecipitates the active kinase. Does not react with most Cdc2 related proteins. Antibody should be titrated for optimal results in individual sample types.
      Biological Information
      Immunogena synthetic peptide (CDNQIKKM) corresponding to the C-terminus of human Cdc2/Cdk1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC25 0

      Documentation

      Anti-Cdc2/Cdk1 (Ab-1) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cdc2/Cdk1 (Ab-1) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC25

      References

      Reference overview
      Reed, S.I., and Wittenberg, C. 1990. Proc. Natl. Acad. Sci. USA 87, 5697.
      Draetta, G., et al. 1987. Cell 50, 319.
      Simanis, V., and Nurse, P. 1986. Cell 45, 261.
      Reed, S.I., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 4055.
      Piggott, J.R., et al. 1982. Nature 298, 391.
      Nurse, P., and Bisset, Y. 1981. Nature 292, 558.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-July-2008 JSW
      SynonymsAnti-Cdk1, Anti-p34
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Application Data
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~34 kDa Cdc2/Cdk1 protein.
      BackgroundThe cdc2 gene was originally isolated using cell division cycle mutants in the fission yeast Schizosaccharomyces pombe. The gene was shown to encode a protein with a molecular weight of ~34 kDa and had all of the properties of protein kinase. The cdc2 gene was subsequently shown to be functionally homologous to the Cdc28 gene in the budding yeast, Saccharomyces cerevisiae, which was demonstrated to encode a protein (serine/threonine) kinase. The Cdc2 kinase is now known to be a key regulator of the eukaryotic cell cycle and is believed to act in both G1 and G2 phases. The activity of the catalytic subunit p34 is controlled by interactions with other proteins, namely the mitotic cyclins and p13suc1, and by phosphorylation-dephosphorylation. The kinase activity of dc2 is most readily studied by using histone H1 as a phosphorylation substrate in vitro. cdc2 is also referred to as cdk1.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (CDNQIKKM) corresponding to the C-terminus of human Cdc2/Cdk1
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlProliferating human or rodent cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsImmunoprecipitates the active kinase. Does not react with most Cdc2 related proteins. Antibody should be titrated for optimal results in individual sample types.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesReed, S.I., and Wittenberg, C. 1990. Proc. Natl. Acad. Sci. USA 87, 5697.
      Draetta, G., et al. 1987. Cell 50, 319.
      Simanis, V., and Nurse, P. 1986. Cell 45, 261.
      Reed, S.I., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 4055.
      Piggott, J.R., et al. 1982. Nature 298, 391.
      Nurse, P., and Bisset, Y. 1981. Nature 292, 558.