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PC253L Anti-Calbindin D-28K (Ab-1) Rabbit pAb

Anti-Calbindin D-28K (Ab-1), rabbit polyclonal, reecognizes the Calbindin D-28K protein in rat hippocampus. It is validated for use in frozen sections and for T cell co-stimulation.

Anti-Calbindin D-28K (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC253L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Bab, H, Mk, RRbPolyclonal Antibody

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PC253L-100UL
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      Description
      OverviewRecognizes the Calbindin D-28K protein in rat hippocampus tissue.
      Catalogue NumberPC253L
      Brand Family Calbiochem®
      References
      ReferencesConde, F., et al. 1994. J. Comp. Neurol. 341, 95.
      Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15.
      Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
      Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat striatum, hippocampus, or cortex
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesFrozen and Vibrotome Sections Raven, M.A., et al. 2005. Vis. Neurosci. 22, 569.
      Key Applications Frozen Sections
      T Cell Co-stimulation
      Application NotesFrozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
      Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
      Application CommentsAntibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified bovine cerebellum calbindin D-28K protein
      ImmunogenBovine
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Baboon
      • Human
      • Monkey
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC253L-100UL 04055977224702

      Documentation

      Anti-Calbindin D-28K (Ab-1) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Calbindin D-28K (Ab-1) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC253L

      References

      Reference overview
      Conde, F., et al. 1994. J. Comp. Neurol. 341, 95.
      Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15.
      Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
      Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.

      Citations

      Title
    • Mary. A. Raven, et al. (2005) Developmental Improvement in the Regularity and Packing of Mouse Horizontal Cells: Implications for Mechanisms Underlying Mosaic Pattern Formation. Visual Neuroscience 22, 569-573.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      ApplicationFrozen Sections (whole mount/vibrotome sections, 1:1000, fluorescence)
      Vibrotome Sections (1:5000-1:10,000 for biotin-streptavidin/peroxidase detection, see application references)
      DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes the calbindin D-28K protein.
      BackgroundSeveral EF-hand calcium-binding proteins have been identified within the nervous system, which includes calbindin, calretinin, and parvalbumin. These cytosolic calcium-binding proteins modulate and mediate the function of the intracellular second messenger calcium. These proteins are believed to function in the buffering, transport and regulation of calcium. Calbindin, calretinin, and parvalbumin are localized in distinct neuronal subpopulations, and antibodies against these proteins are useful markers for neuranatomical and developmental studies.
      HostRabbit
      Immunogen speciesBovine
      Immunogenpurified bovine cerebellum calbindin D-28K protein
      IsotypeIgG
      Speciesbaboon, human, monkey, rat
      Positive controlRat striatum, hippocampus, or cortex
      FormLyophilized
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody specificity was examined in the rat striatum, cortex, and hippocampus. Detection of primary antibody with biotin-streptavidin/peroxidase reagents according to manufacturers directions. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution will contain ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesConde, F., et al. 1994. J. Comp. Neurol. 341, 95.
      Heizmann, C.W. 1993. Acta Neurobiol. Exp. 53, 15.
      Baimbridge, K.G., et al. 1992. Trends Neurosci. 15, 303.
      Heizmann, C.W. and Hunziker, W. 1992. Trends Biochem. Sci. 16, 98.
      Citation
    • Mary. A. Raven, et al. (2005) Developmental Improvement in the Regularity and Packing of Mouse Horizontal Cells: Implications for Mechanisms Underlying Mosaic Pattern Formation. Visual Neuroscience 22, 569-573.
      		•
      Application referencesFrozen and Vibrotome Sections Raven, M.A., et al. 2005. Vis. Neurosci. 22, 569.