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PC389 Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb

This Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb is validated for use in Immunoblotting for the detection of CXCR4 (Ab-1) (182-196).

Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Fusin, Anti-HUMSTR, Anti-LESTR

PC389
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~40 kDa CXCR4 protein in HeLa cells.
      Catalogue NumberPC389
      Brand Family Calbiochem®
      SynonymsAnti-Fusin, Anti-HUMSTR, Anti-LESTR
      Application Data
      Detection of human CXCR4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb (Cat. No. PC389) (1 μg/ml). Detection: chemiluminescence.
      References
      ReferencesBrelot, A., et al. 1997. J. Virol. 71, 4744.
      Dimitrov, D.S. 1997. Cell 91, 721.
      Lu, Z., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6426.
      Berson, J.F., et al. 1996. J. Virol. 70, 6288.
      Bleul, C.C., et al. 1996. Nature 382, 829.
      Doranz, B.J., et al. 1996. Cell 85, 1149.
      Endres, M.J., et al. 1996. Cell 87, 745.
      Feng, Y., et al. 1996. Science 272, 872.
      Lapham, C.K., et al. 1996. Science 274, 602.
      Oberlin, E., et al. 1996. Nature 382, 833.
      Leoetscher. M., et al. 1994. J. Biol. Chem. 269, 232.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmunoblotting Phillips, R.J., et al. 2005. J. Biol. Chem. 280, 22473.
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (2-4µg/ml, see application references)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 182-196 in the second extracellular loop of human CXCR4
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC389 0

      Documentation

      Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC389

      References

      Reference overview
      Brelot, A., et al. 1997. J. Virol. 71, 4744.
      Dimitrov, D.S. 1997. Cell 91, 721.
      Lu, Z., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6426.
      Berson, J.F., et al. 1996. J. Virol. 70, 6288.
      Bleul, C.C., et al. 1996. Nature 382, 829.
      Doranz, B.J., et al. 1996. Cell 85, 1149.
      Endres, M.J., et al. 1996. Cell 87, 745.
      Feng, Y., et al. 1996. Science 272, 872.
      Lapham, C.K., et al. 1996. Science 274, 602.
      Oberlin, E., et al. 1996. Nature 382, 833.
      Leoetscher. M., et al. 1994. J. Biol. Chem. 269, 232.

      Citations

      Title
    • Roderick J. Phillips, et al. (2005) Epidermal Growth Factor and Hypoxia-induced Expression of CXC Chemokine Receptor 4 on Non-small Cell Lung Cancer Cells is Regulated by the Phosphatidylinositol 3-Kinase/PTEN/Akt/Mammalian Target of Rapamycin Signaling Pathway and Activation of Hypoxia Ind. Journal of Biological Chemistry 280, 22473-22481.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-September-2008 RFH
      SynonymsAnti-Fusin, Anti-HUMSTR, Anti-LESTR
      ApplicationImmunoblotting (2-4µg/ml, see application references)
      Application Data
      Detection of human CXCR4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-CXCR4 (Ab-1) (182-196) Rabbit pAb (Cat. No. PC389) (1 μg/ml). Detection: chemiluminescence.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~40 kDa CXCR4 protein.
      BackgroundHuman immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1, and V28 were recently identified as HIV coreceptors1. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The α-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids 182-196 in the second extracellular loop of human CXCR4
      IsotypeIgG
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBrelot, A., et al. 1997. J. Virol. 71, 4744.
      Dimitrov, D.S. 1997. Cell 91, 721.
      Lu, Z., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6426.
      Berson, J.F., et al. 1996. J. Virol. 70, 6288.
      Bleul, C.C., et al. 1996. Nature 382, 829.
      Doranz, B.J., et al. 1996. Cell 85, 1149.
      Endres, M.J., et al. 1996. Cell 87, 745.
      Feng, Y., et al. 1996. Science 272, 872.
      Lapham, C.K., et al. 1996. Science 274, 602.
      Oberlin, E., et al. 1996. Nature 382, 833.
      Leoetscher. M., et al. 1994. J. Biol. Chem. 269, 232.
      Citation
    • Roderick J. Phillips, et al. (2005) Epidermal Growth Factor and Hypoxia-induced Expression of CXC Chemokine Receptor 4 on Non-small Cell Lung Cancer Cells is Regulated by the Phosphatidylinositol 3-Kinase/PTEN/Akt/Mammalian Target of Rapamycin Signaling Pathway and Activation of Hypoxia Ind. Journal of Biological Chemistry 280, 22473-22481.
      		•
      Application referencesImmunoblotting Phillips, R.J., et al. 2005. J. Biol. Chem. 280, 22473.