Millipore Sigma Vibrant Logo

MABF1583 Anti-CD3e (Mouse), PerCP-Cy5.5, clone 145-2C11 Antibody

This armenian hamster monoclonal Anti-CD3e Antibody (Mouse), PerCP-Cy5.5, clone 145-2C11, Cat. No. MABF1584 is validated for use in Flow Cytometry, for the detection of CD3e.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: Cd3e UniProt Number: P22646
View Products on Sigmaaldrich.com
MABF1583
25 μg  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFCAHmPerCP-Cy5.5Monoclonal Antibody
      Description
      Catalogue NumberMABF1583
      DescriptionAnti-CD3e (Mouse), PerCP-Cy5.5, clone 145-2C11 Antibody
      Alternate Names
      • T-cell surface glycoprotein CD3 epsilon chain
      • T-cell surface antigen T3/Leu-4 epsilon chain
      • CD3e
      • CD3e
      Background InformationThe 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).
      Product Information
      FormatPerCP-Cy5.5
      PresentationPurified Armenian Hamster monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
      Quality LevelMQ100
      Applications
      ApplicationThis armenian hamster monoclonal Anti-CD3e Antibody (Mouse), PerCP-Cy5.5, clone 145-2C11, Cat. No. MABF1584 is validated for use in Flow Cytometry, for the detection of CD3e.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      Clone145-2C11
      ConcentrationPlease refer to lot specific datasheet.
      HostArmenian Hamster
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cd3e
      Purification MethodPurified by size-exclusion chromatography
      UniProt Number
      Product Usage Statements
      Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.25 μg from a representative lot detected CD3e in one million C57Bl/6 splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABF1583 04055977292862