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AM53 Anti-Bim Rat mAb (14A8)

Anti-Bim Rat mAb (14A8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bcl-2 Interacting Mediator

AM53
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      R
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~18 kDa Bim, ~20 kDa BimL, and the ~24 kDa BimEL isoforms of Bim in MCF-7 cells.
      Catalogue NumberAM53
      Brand Family Calbiochem®
      SynonymsAnti-Bcl-2 Interacting Mediator
      References
      ReferencesPuthalakath, H., et al. 1999. Mol. Cell 3, 287.
      O’Connor, L., et al. 1998. EMBO J. 17, 384.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlNormal rat IgG
      Positive controlMCF7 cells or Bim overexpressing cell lines
      Preservative≤0.1% sodium azide and 0.05% Bronidox
      Applications
      Application ReferencesImmunblotting O'Reilly et al. 2000. Am. J. Pathol. 157, 449. Kajihara, T. et al. 2006. Mol. Endocrinol. 20, 2444.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (1-2 µg/ml; see application references)
      Immunofluorescence (0.5 µg/ml, see comments)
      Immunoprecipitation (1-2 µg/reaction)
      Application CommentsAnti-Bim recognizes an epitope that is common to all 3 Bim isoforms. Permeabilization of the cell membrane is necessary for Bim detection by immunofluorescence. Bim is a cytoplasmic protein present at low levels; to immunoprecipitate Bim, use 1-5 mg of total cell protein. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, mouse Bim
      ImmunogenMouse
      Clone14A8
      HostRat
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM53 0

      Documentation

      Anti-Bim Rat mAb (14A8) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Bim Rat mAb (14A8) Certificates of Analysis

      TitleLot Number
      AM53

      References

      Reference overview
      Puthalakath, H., et al. 1999. Mol. Cell 3, 287.
      O’Connor, L., et al. 1998. EMBO J. 17, 384.

      Citations

      Title
      Kajihara, T. et al. 2006. Mol. Endocrinol. 20, 2444.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-September-2009 RFH
      SynonymsAnti-Bcl-2 Interacting Mediator
      ApplicationImmunoblotting (1-2 µg/ml; see application references)
      Immunofluorescence (0.5 µg/ml, see comments)
      Immunoprecipitation (1-2 µg/reaction)
      DescriptionPurified rat monoclonal antibody. Recognizes the ~18 kDa Bim, ~20 kDa BimL, and the ~24 kDa BimEL isoforms.
      BackgroundBim, Bcl-2 interacting mediator of cell death, mRNA is differentially spliced to code for 3 isoforms of the Bim protein: Bim-110 amino acids, BimL-140 amino acids and BimEL-196 amino acids. The Bim proteins share a 9 amino acid BH3 motif with bcl-2 homologues and this BH3 region is required for bcl-2 binding. Bim proteins also bind to bcl-xL and bcl-w, but not to Adenovirus E1B19K and Epstein-Barr virus BHRF-1. In healthy cells, BimL binds to the cytoplasmic dynein light chain LC8 and is thereby associated with the microtubule associated dynein motor complex. An apoptotic stimulus that disrupts LC8 and the dynein motor complex allows Bim to interact with and neutralize bcl-2.
      HostRat
      Immunogen speciesMouse
      Immunogenrecombinant, mouse Bim
      Clone14A8
      IsotypeIgG2a
      Specieshuman, mouse
      Positive controlMCF7 cells or Bim overexpressing cell lines
      Negative controlNormal rat IgG
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide and 0.05% Bronidox
      CommentsAnti-Bim recognizes an epitope that is common to all 3 Bim isoforms. Permeabilization of the cell membrane is necessary for Bim detection by immunofluorescence. Bim is a cytoplasmic protein present at low levels; to immunoprecipitate Bim, use 1-5 mg of total cell protein. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesPuthalakath, H., et al. 1999. Mol. Cell 3, 287.
      O’Connor, L., et al. 1998. EMBO J. 17, 384.
      CitationKajihara, T. et al. 2006. Mol. Endocrinol. 20, 2444.
      Application referencesImmunblotting O'Reilly et al. 2000. Am. J. Pathol. 157, 449. Kajihara, T. et al. 2006. Mol. Endocrinol. 20, 2444.