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PC68 Anti-Bcl-2 (Ab-2) (20-34) Rabbit pAb

Anti-Bcl-2 (Ab-2) (20-34) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC68
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, OpRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~24-26 kDa Bcl-2 protein in MCF-7 cells, intestine, and lymph node tissue.
      Catalogue NumberPC68
      Brand Family Calbiochem®
      References
      ReferencesKozopas, K.M. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
      Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
      Silverman, G.A., et al. 1993. Mol. Cell. Biol. 13, 5469.
      Vaux, D.L. 1993. Proc. Natl. Acad. Sci.USA 90, 786.
      Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
      Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
      Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
      Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
      Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Negative controlBrain tissue
      Positive controlHuman intestine or lymph node tissue or purified Bcl-2 (Cat. No. PF126)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen sections (5 µg/ml)
      Immunoblotting (5 µg/ml)
      Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
      Application CommentsBrain tissue may be used as a negative control, as only random cells stain positively. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (HYKLSQRGYEWDAGD) corresponding to amino acids 20-34 of human Bcl-2
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Opossum
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC68 0

      Documentation

      Anti-Bcl-2 (Ab-2) (20-34) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC68

      References

      Reference overview
      Kozopas, K.M. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
      Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
      Silverman, G.A., et al. 1993. Mol. Cell. Biol. 13, 5469.
      Vaux, D.L. 1993. Proc. Natl. Acad. Sci.USA 90, 786.
      Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
      Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
      Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
      Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
      Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.

      Citations

      Title
    • Denis Grandgirard, et al. (1998) Alphaviruses induce apoptosis in Bcl-2-overexpressing cells: evidence for a caspase-mediated, proteolytic inactivation of Bcl-2. The EMBO Journal 17, (1268- 1278).
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-February-2008 JSW
      ApplicationFrozen sections (5 µg/ml)
      Immunoblotting (5 µg/ml)
      Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~24-26 kDa Bcl-2 protein.
      BackgroundThe bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a 24-26 kDa protein which has been localized to the inner mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death." It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock, and irradiation.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (HYKLSQRGYEWDAGD) corresponding to amino acids 20-34 of human Bcl-2
      IsotypeIgG
      Specieshuman, mouse, opossum
      Positive controlHuman intestine or lymph node tissue or purified Bcl-2 (Cat. No. PF126)
      Negative controlBrain tissue
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsBrain tissue may be used as a negative control, as only random cells stain positively. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKozopas, K.M. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
      Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
      Silverman, G.A., et al. 1993. Mol. Cell. Biol. 13, 5469.
      Vaux, D.L. 1993. Proc. Natl. Acad. Sci.USA 90, 786.
      Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
      Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
      Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
      Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
      Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
      Citation
    • Denis Grandgirard, et al. (1998) Alphaviruses induce apoptosis in Bcl-2-overexpressing cells: evidence for a caspase-mediated, proteolytic inactivation of Bcl-2. The EMBO Journal 17, (1268- 1278).
      		•