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MAB5372 Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31

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MAB5372
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Gp, H, M, RWB, ICC, IH(P)MAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB5372
      ReplacesMAB5314
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31
      Product Information
      FormatAscites
      PresentationAscites fluid. Liquid.
      Quality LevelMQ100
      Applications
      ApplicationDetect Astrocytomas using this Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 validated for use in WB, IC, IH(P).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: reducing conditions, recognizes a 30kDa protein {Singh, R et al (1986) Biosci Rep 6(1):73-80.

      Immunohistochemistry The antibody works on paraffin embedded tissue sections.

      Immunocytochemistry

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman cerebral white matter plaque materials from a multiple sclerosis patient.
      Epitopea marker for Low Grade Astrocytoma
      CloneJ1-31
      HostMouse
      SpecificityThe antibody recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS).The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue. The antibody is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed).

      Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP {Malhotra, SK (1989) J Neurosci Res. 22(1):36-49}.
      IsotypeIgM
      Species Reactivity
      • Guinea Pig
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5372 04053252464003