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About This Item
Product Name
Amicon® Ultra Centrifugal Filter, 100 kDa MWCO, sample volume 2 mL, regenerated cellulose membrane, MWCO 100 kDa
material
polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
2 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
119.7 mm
diam.
15.9 mm
filtration area
1 cm2
volume
2 mL
working volume
≤2 mL
2 mL
pore size
100 kDa MWCO
Quality Level
greener alternative category
shipped in
ambient
Application
- Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates.
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 2 mL down to 15-70 μL
- 98% sample recovery
- Fast processing time: 10 to 30-minute spin times
- Engineered dead stop
- Reverse spin capability provides consistent recoveries
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.
Other Notes
Physical form
Legal Information
Certificates of Analysis (COA)
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Related Content
"The Amicon® Ultra 2 mL filter provides a fast and easy way to concentrate, desalt and exchange thebuffer for both proteins and nucleic acids. Now you can concentrate 2 mL down to 15 – 70 μL in anAmicon® Ultra centrifugal device."
Global Trade Item Number
| SKU | GTIN |
|---|---|
| UFC210024 | 04053252691812 |
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