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129935 Actinomycin D, 7-Amino- - CAS 7240-37-1 - Calbiochem

Actinomycin D, 7-Amino, CAS 7240-37-1, is a DNA intercalator that complexes with DNA. Absorbs at 555 nm & emits at 655 nm. Exhibits growth-inhibitory activity against certain leukemias and sarcomas.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 7-AAD, 7-Amino-AMD, 7-Aminoactinomycin C₁, 7-Aminodactinomycin

Primary Target: leukemias and sarcomas
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      Overview

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      7240-37-1C₆₂H₈₇N₁₃O₁₆
      Description
      Overview7-Aminoactinomycin D, like its parent molecule, actinomycin D, is a DNA intercalator and has antibacterial properties. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. Fluorescent excitation and emission maxima are 500 and 672 nm, respectively. Complexes with DNA and absorbs at 555 nm and emits at 655 nm. Useful in distinguishing early apoptotic cells, which have lost membrane integrity, from apoptotic and live cells. Has been used in DNA analysis and as a probe for chromosome structure and function.
      Catalogue Number129935
      Brand Family Calbiochem®
      Synonyms7-AAD, 7-Amino-AMD, 7-Aminoactinomycin C₁, 7-Aminodactinomycin
      References
      ReferencesSchmid, I., et al. 1994. J. Immunol. Methods 170, 145.
      Berg, R.J., et al. 1993. Carcinogenesis 14, 103.
      Chen, F.M. 1992. Biochemistry 31, 6223.
      Schmid, I., et al. 1992. Cytometry 13, 204.
      Toba, K., et al. 1992. Cytometry 13, 60.
      Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
      Product Information
      CAS number7240-37-1
      FormReddish-purple solid
      Hill FormulaC₆₂H₈₇N₁₃O₁₆
      Chemical formulaC₆₂H₈₇N₁₃O₁₆
      Hygroscopic Hygroscopic
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      ApplicationActinomycin D, 7-Amino, CAS 7240-37-1, is a DNA intercalator that complexes with DNA. Absorbs at 555 nm & emits at 655 nm. Exhibits growth-inhibitory activity against certain leukemias and sarcomas.
      Biological Information
      Primary Targetleukemias and sarcomas
      Purity≥85% by HPLC
      Sample TypeIntact Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSAU1579000
      Safety Information
      R PhraseR: 28

      Very toxic if swallowed.
      S PhraseS: 36/37-45-28

      Wear suitable protective clothing and gloves.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Highly Toxic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot, purge with inert gas and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      129935 0

      Documentation

      Actinomycin D, 7-Amino- - CAS 7240-37-1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Actinomycin D, 7-Amino- - CAS 7240-37-1 - Calbiochem Certificates of Analysis

      TitleLot Number
      129935

      References

      Reference overview
      Schmid, I., et al. 1994. J. Immunol. Methods 170, 145.
      Berg, R.J., et al. 1993. Carcinogenesis 14, 103.
      Chen, F.M. 1992. Biochemistry 31, 6223.
      Schmid, I., et al. 1992. Cytometry 13, 204.
      Toba, K., et al. 1992. Cytometry 13, 60.
      Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.

      Citations

      Title
    • Jeffrey D. Price, et al. (2005) Induction of a regulatory phenotype in human CD4+ T cells by Streptococcal M protein. Journal of immunology 175, 677-684.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-August-2010 JSW
      Synonyms7-AAD, 7-Amino-AMD, 7-Aminoactinomycin C₁, 7-Aminodactinomycin
      Description7-Aminoactinomycin D is a membrane impermeable fluorescent DNA intercalator. Like its parent compound, Actinomycin D (Cat. No. 114666), 7-AAD has antibacterial properties and growth inhibitory activity against certain leukemias and sarcomas. Useful for flow cytometry and fluorescence microscopy. Used for staining nonviable cells and for identifying apoptotic cells. Compatible with FITC and PE cell surface staining. The absorption/emission maxima are as follows: unbound (ex. 500 nm, em. 672 nm) and DNA bound (ex. 555 nm, em. 655 nm). λmax 528 nm ± 2; εmax = 22,700 ± 5,000 M-1 cm-1
      FormReddish-purple solid
      CAS number7240-37-1
      RTECSAU1579000
      Chemical formulaC₆₂H₈₇N₁₃O₁₆
      Structure formulaStructure formula
      Purity≥85% by HPLC
      SolubilityDMSO (20 mg/ml) or Ethanol (5 mg/ml). Further dilute in aqueous buffers just prior to use.
      Storage Protect from light
      -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot, purge with inert gas and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
      Toxicity Highly Toxic
      Merck USA index14, 2800
      ReferencesSchmid, I., et al. 1994. J. Immunol. Methods 170, 145.
      Berg, R.J., et al. 1993. Carcinogenesis 14, 103.
      Chen, F.M. 1992. Biochemistry 31, 6223.
      Schmid, I., et al. 1992. Cytometry 13, 204.
      Toba, K., et al. 1992. Cytometry 13, 60.
      Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
      Citation
    • Jeffrey D. Price, et al. (2005) Induction of a regulatory phenotype in human CD4+ T cells by Streptococcal M protein. Journal of immunology 175, 677-684.
      		•