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108512 AFB-Color carbol fuchsin solution

for the microscopic investigation of acid-fast bacteria (AFB) (cold staining)

AFB-Color carbol fuchsin solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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108512
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      		 Plastic bottle 500 ml
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      1085122500
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          		 Plastic bottle 2.5 l
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          Description
          Catalogue Number108512
          DescriptionAFB-Color carbol fuchsin solution
          OverviewThe AFB-Color carbol fuchsin solution - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining), is a ready-to- use solution used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. The AFB-Color Kit (Cat. no. 116450) offers a modified AFB-Color Carbol fuchsin solution which abolishes heating (Kinyoun method, cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even after intense treatment with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use 110630 AFB-Color malachite green (oxalate) solution or 101287 Loeffler's methylene blue is used for counterstaining.
          The 500 ml bottle of AFB-Color carbol fuchsin provides approx. 250 stainings.
          Pretreatment of the specimens with Sputofluol®(Cat. No. 108000) dissolves the bacteria from the surrounding viscid sputum and cell material. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          Product Information
          HS Code3212 90 90
          Quality LevelMQ400
          Applications
          Applicationfor the microscopic investigation of acid-fast bacteria (AFB) (cold staining)
          Physicochemical Information
          Density0.99 g/cm3 (20 °C)
          Flash point48 °C
          pH value3.6 (H₂O, 20 °C)
          Safety Information according to GHS
          Hazard Statement(s)H226: Flammable liquid and vapour.
          H314: Causes severe skin burns and eye damage.
          H341: Suspected of causing genetic defects.
          H410: Very toxic to aquatic life with long lasting effects.
          Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
          P273: Avoid release to the environment.
          P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
          P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
          P304 + P340 + P310: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor.
          P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
          Storage class3 Flammable liquids
          WGKWGK 3 highly hazardous to water
          Disposal9
          Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
          Safety Information
          Categories of dangerflammable, harmful, corrosive, mutagenic
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Transport Information
          Declaration (railroad and road) ADR, RIDUN 1992 , 3 (6.1), III
          Declaration (transport by air) IATA-DGRUN 1992 , 3 (6.1), III
          Declaration (transport by sea) IMDG-CodeUN 1992 , 3 (6.1), III
          Global Trade Item Number
          Catalogue Number GTIN
          1085120500 04022536105888
          1085122500 04022536557175