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480706 α2,3-Neuraminidase, Macrobdella decora, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Sialidase L

480706
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, Macrobdella decora α2,3-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of α2,3-linked N-acetylneuraminic acid from complex oligosaccharides. Will catalyze the cleavage of α2,3-linkages from α1-acid glycoprotein, fetuin, keratan sulfate, sialyl Lewis x, and 3′-sialyllactose, yielding 2,7-anhydro-Neu5Ac instead of “free Neu5Ac” as the hydrolysis product.
      Note: 1 mU = 1 milliunit.
      Catalogue Number480706
      Brand Family Calbiochem®
      SynonymsSialidase L
      References
      ReferencesChou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.
      Product Information
      CAS number9001-67-6
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 nmol methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.5.
      EC number3.2.1.18
      FormLiquid
      FormulationIn 10 mM phosphate buffer, 2% glycerol, pH 7.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥2000 U/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsCeramide glycanase, α- and β-galactosidase, β-hexosaminidase, α- and β-mannosidase, proteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSQQ3450000
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      480706 0

      Documentation

      α2,3-Neuraminidase, Macrobdella decora, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      480706

      References

      Reference overview
      Chou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-June-2009 JSW
      SynonymsSialidase L
      DescriptionRecombinant, Macrobdella decora α2,3-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of α2,3-linked N-acetylneuraminic acid from complex oligosaccharides. Will catalyze the cleavage of α2,3-linkages from α1-acid glycoprotein, fetuin, keratan sulfate, sialyl Lewis x, and 3'-sialyllactose, yielding 2,7-anhydro-Neu5Ac instead of "free Neu5Ac" as the hydrolysis product.
      FormLiquid
      FormulationIn 10 mM phosphate buffer, 2% glycerol, pH 7.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      CAS number9001-67-6
      RTECSQQ3450000
      EC number3.2.1.18
      ContaminantsCeramide glycanase, α- and β-galactosidase, β-hexosaminidase, α- and β-mannosidase, proteases: none detected
      Specific activity≥2000 U/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 nmol methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.5.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Harmful
      ReferencesChou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.