On-Demand Webinar

Clarification of Mammalian Cell Culture Feed Streams Using a Cationic Flocculant, pDADMAC, and Clarisolve® Depth Filters

Duration: 1 Hour
Speaker: Nripen Singh, Senior Research Scientist, Merck

Abstract: Increasing cell culture densities and productivities during therapeutic protein (MAbs) production are placing a larger burden on downstream clarification and purification operations due to higher product and impurity levels. Traditionally, centrifugation and a combination of filtration techniques (tangential-flow filtration and depth filtration) have been widely used for clarifying these pretreated cell culture broths. However, the increased cellular debris present in these complex feed streams can prematurely foul the membrane and commercially available depth filters, adversely impacting their capacity and throughput. In this work, Merck has developed a cationic flocculant, Polydiallyldimethylammonium chloride (pDADMAC), as an effective pretreatment method for mammalian cell culture harvest which when coupled with Clarisolve® depth filters result in improved primary and secondary clarification for flocculated feeds. Potential benefits will be discussed, including more robust processing with a centrifuge and the potential to use filters in place of a centrifuge.

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