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Rapid Testing of Clostridium Perfringens

 
 

Reliable Testing Solutions for the
Detection of Clostridium Perfringens

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Clostridium perfringens is an anaerobic, spore-forming and toxin-producing bacterium which can cause intense abdominal cramps, diarrhea and - in rare cases - necrotic enteritis (pig-bel syndrome). Small cell numbers may be present after cooking and multiply to food poisoning levels during cool down and storage of prepared foods. Typically, Clostridium perfringens will grow in foods that are high in starch or protein, such as cooked beans, meat products, thick soups, and gravy.

Merck offers a selection of products for the enrichment, sample dilution, detection and isolation of Clostridium perfringens. The range includes dehydrated culture media in a unique and user-friendly granulated format. These meet the highest industry standards for performance as laid out in ISO 11133.

Method ISO 7937 FDA BAM Chapter 16 FSIS MLG 13.00 /
FDA BAM Chapter 16
MLG 5.05
Isolation FSIS MLG 13.00 /
FDA BAM Chapter 16
Sulfite-Cycloserine Agar (TSC w/o egg yolk),
35°C;
20 ± 2 h,
anaerobic
TSC Agar w/ egg yolk, overlay w/o egg yolk, 35°C; 24 h, anaerobic
Confirmation Fluid Thioglycollate medium,
37°C / 35°C, 21 ±
3 h, anaerobic
Lactose Sulfite medium, 46°C,
21 ± 3 h / Nitrate Motility medium, 37°C, 24 h, anaerobic
Fluid Thioglycollate medium,
35°C,
21 ± 3 h,
Nitrate Motility medium,
35°C, 24 h,
anaerobic
Fluid Thioglycollate medium,
35°C,
21 ± 3 h
Nitrate Motility medium,
35°C, 24 h,
anaerobic
 
 
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