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70765 pET Expression System 22b - Novagen

70765
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概要

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70765-3CN
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      Description
      OverviewpET Expression Systems and pET
      pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

      Components: pET Expression Systems
      Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
      • 10 µg pET vector DNA (for each indicated plasmid)
      • 0.2 ml BL21 Glycerol Stock
      • 0.2 ml BL21(DE3) Glycerol Stock
      • 0.2 ml BL21(DE3)pLysS Glycerol Stock
      • 0.2 ml Induction Control Glycerol Stock

      Components: pET Expression Systems plus Competent Cells
      pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
      • 0.2 ml NovaBlue Competent Cells
      • 0.2 ml BL21(DE3) Competent Cells
      • 0.2 ml BL21(DE3)pLysS Competent Cells
      • 2 × 0.2 ml SOC Medium
      • 10 µl Test Plasmid

      These components are sufficient for up to 10 transformations in each host.

      Purification and Detection Reagents
      Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

      pET Expression System 22b
      The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB038VM.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number70765
      Brand Family Novagen®
      References
      Product Information
      Components
      Fusion tagHis•Tag
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      カタログ番号 GTIN
      70765-3CN 04055977273182

      Documentation

      pET Expression System 22b - Novagen 試験成績書(CoA)

      タイトルロット番号
      70765

      引用

      Title
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    • Bogdan Yatsula, et al. (2005) Identification of binding sites of EVI1 in mammalian cells. Journal of Biological Chemistry 280, 30712-30722.
    • Heyue Zhou, et al. (2005) Solution structure of AF-6 PDZ domain and its interaction with the C-terminal peptides from neurexin and Bcr. Journal of Biological Chemistry 280, 13841-13847.
    • Guoqiang Zhu, et al. (2005) Histone H1 proteins act as receptors for the 987P fimbriae of enterotoxigenic Escherichia coli. Journal of Biological Chemistry 280, 23057-23065.
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    • Hiroyasu Furukawa and Eric Gouaux. (2003) Mechanisms of activation, inhibition and specifcity: crystal structures of the NMDA receptor NR1ligand-binding core. European Molecular Biology Organization Journal 22, 2873-2885.
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    • ユーザープロトコール

      タイトル
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      ベクターマップ

      タイトル
      TB038VM pET-22b(+) Vector Map

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      カテゴリー

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors