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Buffer Detection Kit for Magnetic Beads
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ST1629
Sigma-AldrichAnti-GNA13 Mouse mAb (6F6-B5)
Anti-GNA13, mouse monoclonal, clone 6F6-B5, recognizes the ~40-45 kDa GNA13 protein in HepG2 cells. It is validated for use in ELISA and Western blotting.
More>>Anti-GNA13, mouse monoclonal, clone 6F6-B5, recognizes the ~40-45 kDa GNA13 protein in HepG2 cells. It is validated for use in ELISA and Western blotting. Less<<
Anti-GNA13 Mouse mAb (6F6-B5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
Full-length, recombinant, human GNA13 (aa 1-378), expressed as a GST fusion protein
Clone
6F6-B5
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-November-2010 RFH
Synonyms
Anti-G Protein α13, Anti-Guanine Nucleotide Binding Protein α13
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human GNA13 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-GNA13 Mouse mAb (6F6-B5) (Cat. No. ST1629) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa GNA13 protein.
Background
Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an α-, β- and γ-subunit and is bound to GDP in the 'off' state. Ligand binding to a 7-TM receptor causes a conformational change in the receptor, which detaches the G protein. This reaction switches the G protein to an 'on' state, allowing GTP to displace GDP, thus Gα can activate second messenger signaling cascades. There are several types of Gα proteins; Gαs and Gαi bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively. Gαq/11 stimulates PLCβ which catalyzes the hydroylsis of PIP2 to the second messengers IP3 and DAG. Gv12/13 are involved in Rho family GTPase signaling. In addition, some Gβγ subunits have active functions; Gβv coupled to H1 receptors can activate PLA2 and Gβγ coupled to M1 receptors can activate KIR channels. Rapid termination of the cellular response is required once the concentration of the stimulating ligand decreases. GTP bound to Gα is hydrolyzed rapidly to GDP, Gα is converted to its inactive form and re-associates with the β- and γ-subunits returning to the 'off' state.
Host
Mouse
Immunogen
Full-length, recombinant, human GNA13 (aa 1-378), expressed as a GST fusion protein
Clone
6F6-B5
Isotype
IgG₁
Species
human
Positive control
HepG2 cells
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
