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The following MAPmates™ should not be plexed together:
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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ST1622
Sigma-AldrichAnti-LEPRE1 Mouse mAb (3C7)
This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1.
More>>This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1. Less<<
Anti-LEPRE1 Mouse mAb (3C7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Detection of human LEPRE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (1-5 µg/ml). Detection: chemiluminescence. Detection of human LEPRE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (10 µg/ml). Detection: fluorescence. Detection of human LEPRE1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human esophagus tissue. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (6 µg/ml). Detection: colorimetric (DAB).
References
References
Choi, JW, et al. 2009. PLoS Genet.5, e1000750. Cabral, W,A., et al. 2007. Nat Genet.39, 359.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
Clone
3C7
Host
Mouse
Isotype
IgG2a
Antibody Type
Monoclonal Antibody
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Choi, JW, et al. 2009. PLoS Genet.5, e1000750. Cabral, W,A., et al. 2007. Nat Genet.39, 359.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of human LEPRE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (1-5 µg/ml). Detection: chemiluminescence. Detection of human LEPRE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (10 µg/ml). Detection: fluorescence. Detection of human LEPRE1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human esophagus tissue. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (6 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.
Background
This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Two alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
Clone
3C7
Isotype
IgG2a
Positive control
HeLa cells, Human esophagus tissue
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Regulatory Review
References
Choi, JW, et al. 2009. PLoS Genet.5, e1000750. Cabral, W,A., et al. 2007. Nat Genet.39, 359.
