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48-602MAG
Buffer Detection Kit for Magnetic Beads
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ST1533
Sigma-AldrichAnti-ALDH2 Mouse mAb (1E5)
Anti-ALDH2, mouse monoclonal, clone 1E5, recognizes the ~50 kDa ALDH2 protein in MCF-7 cells. It is validated for ELISA and Western blotting.
More>>Anti-ALDH2, mouse monoclonal, clone 1E5, recognizes the ~50 kDa ALDH2 protein in MCF-7 cells. It is validated for ELISA and Western blotting. Less<<
Anti-ALDH2 Mouse mAb (1E5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 408-517 of human ALDH2, expressed as a GST fusion protein
Clone
1E5
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-September-2016 JSW
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human ALDH2 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-ALDH2 Mouse mAb (1E5) (Cat. No. ST1533) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~50 kDa ALDH2 protein.
Background
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while ~50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids 408-517 of human ALDH2, expressed as a GST fusion protein
Clone
1E5
Isotype
IgG2a
Species
human
Positive control
MCF7 cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
