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SCR047 Pancreatic Cell DTZ Detection Assay

The Pancreatic Cell DTZ Detection Assay provides researcher a simple & quick method to identify insulin-producing beta cells from a mixed cell culture preparation or from pancreatic tissues.

Pancreatic Cell DTZ Detection Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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SCR047
1 kit  
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      Overview

      Key Spec Table

      Detection Methods
      Chromogenic
      Description
      Catalogue NumberSCR047
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionPancreatic Cell DTZ Detection Assay
      OverviewThe Pancreatic Cell DTZ Detection Assay provides researcher a simple and quick method to identify insulin-producing beta cells from a mixed cell culture preparation or from pancreatic tissues The kit contains pre-mixed Dithizone Stain, Rinsing Solution and filters for fast preparation and optimized results. Millipore's Pancreatic Cell DTZ Detection Asssay (SCR047) takes advantage of the high zinc content of pancreatic beta cells and the known action of dithizone, a zinc-chelating agent, to provide a quick and simple enzymatic reaction that allows researcher to reliably identify insulin-producing beta cells from a mixed cell culture preparation or from pancreatic tissues. Along with selectively staining live pancreatic beta-islet cells crimson red, the enzymatic reaction has the advantage of being completely reversible. Kit components include staining and rinse solutions along with filters required for the live staining reactions. Sufficient reagents are supplied to provide for 10 enzymatic reactions at 10 mL volume.
      Background InformationThe islets of Langerhans are groups of specialized cells that are dispersed throughout the pancreas. They produce a number of hormones including glucagon, insulin, Pancreatic polypeptide and somatostatin. Insulin is the most critical of these, as it helps regulate glucose metabolism. Insulin is synthesized from a precursor molecule, proinsulin, in the beta cells of the islets of Langerhans. The conversion of proinsulin into the active hormone, insulin, is accomplished by proteolytic enzymes known as prohormone convertases (PC1 and PC2) as well as the exoprotease carboxypeptidase E. These proteolytic enzymes remove the center portion of the molecule, or C-peptide, and the remaining peptide chains (A and B) are cross-linked by disulfide bonds. The crosslinked peptides are the insulin monomer. As the concentration of the protein increases, the monomers assemble into dimers and in the presence of zinc, into hexameric crystals characterized by two Zn2+ atoms per hexameric unit. It is this 2-Zn insulin hexamer that represents the active conformation of the secreted hormone. Given its prominent role in the synthesis, storage and secretion of insulin, it is not surprising that zinc is highly expressed in pancreatic beta cells.
      Product Information
      Components
      • 1 mL of 100X DTZ Stain Solution (Part No: 2006182)
      • 2 - 150 mL of 1X DTZ Rinse Solution (Part No: 2006183)
      • 10 Millex GP 0.22 uM filters (Part No: SLGPR33RB)
      • 12 Plastic Syringes, 20 mL w/ Luer slip (Part No: XX1102012)
      Detection methodChromogenic
      Quality LevelMQ100
      Applications
      ApplicationThe Pancreatic Cell DTZ Detection Assay provides researcher a simple & quick method to identify insulin-producing beta cells from a mixed cell culture preparation or from pancreatic tissues.
      Biological Information
      Species Reactivity
      • Mammals
      Stem Cell Type
      • Pancreatic Stem Cells
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore DTZ Stain at -20C. Store all other reagents at room temperature.
      Packaging Information
      Material Size1 kit
      Global Trade Item Number
      Catalogue Number GTIN
      SCR047 04053252009501