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PH03L Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D)

Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Protein Tyrosine Phosphatase, T cell

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PH03L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the T-cell PTPase protein.
      Catalogue NumberPH03L
      Brand Family Calbiochem®
      SynonymsAnti-Protein Tyrosine Phosphatase, T cell
      References
      ReferencesBrown-Shimer, S., et al. 1992. Can. Res. 52, 478.
      Fischer, E.H., et al. 1991 Science 253, 401.
      Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA 87, 5148.
      Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
      Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 7280.
      Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
      Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
      Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM sodium bicarbonate, 100 µg BSA.
      Positive controlCCRF-CEM cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunocytochemistry (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Application CommentsThis antibody has been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human T cell PTPase
      ImmunogenHuman
      CloneCF4-1D
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4 to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PH03L 0

      Documentation

      Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) Certificates of Analysis

      TitleLot Number
      PH03L

      References

      Reference overview
      Brown-Shimer, S., et al. 1992. Can. Res. 52, 478.
      Fischer, E.H., et al. 1991 Science 253, 401.
      Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA 87, 5148.
      Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
      Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 7280.
      Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
      Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
      Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.

      Brochure

      Title
      Protein Phosphatases Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      SynonymsAnti-Protein Tyrosine Phosphatase, T cell
      ApplicationImmunocytochemistry (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the PTPase protein.
      BackgroundProtein tyrosine phosphatases (PTPases) make up an expanding family of transmembrane and intracellular proteins, which function to remove phosphate groups from protein phosphoryl- tyrosine residues. The prototype phosphatase, PTPase 1B, was initially identified as an active intracellular phosphatase from human placenta made up of 321 amino acids and subsequently shown to derive from a larger precursor protein containing 435 amino acids. Using probes to the conserved PTPase domain a cDNA clone was isolated from a human peripheral T cell library encoding a protein with 65% identity to the placental PTPase 1B. The T cell protein differs in amino acid composition but is highly similar with respect to hydrophobicity with the 1B protein and is now known to be present in other cell types. While the function of the T cell PTPase protein is still unknown, recent experiments demonstrated that a truncated form overexpressed in BHK cells resulted in a greatly reduced growth rate of the cells. PTPases are involved in regulating processes where protein kinases and protein tyrosine phosphorylation are important. One intriguing hypothesis is that PTPases have the potential to function as anti-oncogenes.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human T cell PTPase
      CloneCF4-1D
      IsotypeIgG2a
      Specieshuman, not mouse
      Positive controlCCRF-CEM cells
      FormLyophilized
      FormulationLyophilized from 20 mM sodium bicarbonate, 100 µg BSA.
      PreservativeNone
      CommentsThis antibody has been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4 to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesBrown-Shimer, S., et al. 1992. Can. Res. 52, 478.
      Fischer, E.H., et al. 1991 Science 253, 401.
      Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA 87, 5148.
      Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
      Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 7280.
      Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
      Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
      Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.