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PF048 Heregulin, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Neu Differentiation Marker, NDF

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PF048
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.



      Recombinant, human heregulin expressed in E. coli. Heregulins have been shown to be mitogenic for Schwann cells and various epithelial cells. It is recommended that the protein be titrated for optimal results in individual systems.
      Catalogue NumberPF048
      Brand Family Calbiochem®
      SynonymsNeu Differentiation Marker, NDF
      References
      ReferencesRiese, D.J., et al. 1996. J. Biol. Chem. 271, 20047.
      Holmes, W.E., et al. 1992. Science 256, 1205.
      Karey, K.P., et al. 1988. Cancer Res. 48, 4083.
      Product Information
      FormLyophilized
      FormulationLyophilized from PBS, 50 µg BSA/µg cytokine.
      Quality LevelMQ100
      Applications
      Data setup Error. Pagelet <collection_feature_application_id-PROLIF> not found.
      Application NotesProliferation Studies
      Application CommentsIt is recommended that the protein be titrated for optimal results in individual systems.
      Biological Information
      Biological activityEC₅₀ ~20-40 ng/ml as determined by a cell proliferation assay using breast carcinoma cell line SK-BR-3 or in a serum-free cell proliferation assay using the human cell line MCF7.
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsStore lyophilized protein dessicated at -20°C. Lyophilized product should be reconstituted in sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 50 µg/ml. Following initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PF048 0

      Documentation

      Heregulin, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Heregulin, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      PF048

      References

      Reference overview
      Riese, D.J., et al. 1996. J. Biol. Chem. 271, 20047.
      Holmes, W.E., et al. 1992. Science 256, 1205.
      Karey, K.P., et al. 1988. Cancer Res. 48, 4083.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsNeu Differentiation Marker, NDF
      ApplicationProliferation Studies
      DescriptionRecombinant, human heregulin expressed in E. coli. Human recombinant heregulin whose DNA sequence encoding the EGF domain of heregulin α (amino acids 177-241) expressed in and purified from E. coli. The expressed protein consists of 65 amino acids with a predicted molecular weight of approximately 7 kDa.
      BackgroundIn addition to the well-studied neurotrophic factors and their receptors, many other growth factors and cytokines have been identified which exhibit proliferative or neuroprotective effects. Heregulin is one member of a family of structurally related polypeptides, which includes the epidermal growth factor, transforming growth factor-alpha, cripto-1, amphiregulin, and several viral growth factors. Heregulin has been shown to mediate growth regulatory functions through the epidermal growth factor receptor.
      FormLyophilized
      FormulationLyophilized from PBS, 50 µg BSA/µg cytokine.
      Purity≥97% by SDS-PAGE
      Biological activityEC₅₀ ~20-40 ng/ml as determined by a cell proliferation assay using breast carcinoma cell line SK-BR-3 or in a serum-free cell proliferation assay using the human cell line MCF7.
      CommentsIt is recommended that the protein be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsStore lyophilized protein dessicated at -20°C. Lyophilized product should be reconstituted in sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 50 µg/ml. Following initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesRiese, D.J., et al. 1996. J. Biol. Chem. 271, 20047.
      Holmes, W.E., et al. 1992. Science 256, 1205.
      Karey, K.P., et al. 1988. Cancer Res. 48, 4083.