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PC421 Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TRPV2

PC421
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~98 kDa (doublet) vanilloid receptor like protein-1 in rat spinal cord extract. Supplied with a control peptide (100 µg size only).
      Catalogue NumberPC421
      Brand Family Calbiochem®
      SynonymsAnti-TRPV2
      Application Data
      Detection of mouse vanilloid-receptor-like protein 1 by staining frozen sections. Sample: Mouse spinal cord dorsal horn fixed in 4% paraformaldehyde. Primary antibody: Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb (Cat. No. PC421) (1 µg/ml). Staining was completely abolished by preincubating the purified antibody with control peptide at 10-6 M (data not shown).

      Detection of rat vanilloid-receptor-like protein 1 by immunoblotting. Sample: Whole tissue extract from rat spinal cord pre-incubated without peptide (lane 1) or with control peptide (25 µg/ml, lane 2). Primary antibody: Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb (Cat. No. PC421) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesStrokes, A.L., et al., 2004. J. Exp. Med. 200, 137.
      Caterina, M.J., et al., 1999. Nature 398, 436.
      Gau, A., et al., 1999. Eur. J. Neurosci. 11, 946.
      Michael, G.J. and Priestley, J.V., 1999. J. Neurosci. 19, 1844.
      Caterina, M.J., et al., 1997. Nature 389, 816.
      Szallasi, A., 1994. Gen. Pharmacol. 25, 223.
      Beven, S., and Szolcsanyi, J., 1990. Trends Pharmacol. Sci. 11, 330.
      Fields, H.L., 1987. Pain (New York: McGraw-Hill), 1987.
      Product Information
      FormLiquid
      Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide (immunogen) (100 µg size only).
      Positive controlRat or mouse spinal cord extract
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting and Immunocytochemistry Jahnel, R., et al. 2003. Eur. J. Biochem. 270, 4264.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesFrozen Sections (1 µg/ml)
      Immunoblotting (2 µg/ml, see application references)
      Immunocytochemistry (2 µg/ml, see application references)
      Application CommentsA protein database search confirms that this peptide sequence is unique to the Vanilloid-Receptor-Like Protein 1. No cross-reactivity with the capsaicin receptor protein is expected. This signal is completely abolished in immunoblotting by preincubating the affinity purified antibody with peptide at 25 µg/ml. Unconjugated Vanilloid-Receptor-Like Protein 1 Control Peptide has been included for use as an internal control for antibody specificity. Immunohistochemical staining results correlate well with other independent reports. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (KNSASEEDHLPLQVLQSP) corresponding to amino acids 744-761 of rat vanilloid receptor like protein 1, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Special InstructionsReconstitute the control peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Store lyophilized control peptide at -20°C. Following reconstitution of the control peptide, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC421 0

      Documentation

      Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC421

      References

      Reference overview
      Strokes, A.L., et al., 2004. J. Exp. Med. 200, 137.
      Caterina, M.J., et al., 1999. Nature 398, 436.
      Gau, A., et al., 1999. Eur. J. Neurosci. 11, 946.
      Michael, G.J. and Priestley, J.V., 1999. J. Neurosci. 19, 1844.
      Caterina, M.J., et al., 1997. Nature 389, 816.
      Szallasi, A., 1994. Gen. Pharmacol. 25, 223.
      Beven, S., and Szolcsanyi, J., 1990. Trends Pharmacol. Sci. 11, 330.
      Fields, H.L., 1987. Pain (New York: McGraw-Hill), 1987.

      Brochure

      Title
      Stem Cell Resource Brochure GBP

      Citations

      Title
    • Math P. Cuajungco, et al. (2006) PACSINs bind to the TRPV4 cation channel: PACSIN 3 modulates the subcellular localization of TRPV4. Journal of Biological Chemistry in press,.
    • Ricarda Jahnel, et al. (2003) Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression. European Journal of Biochemistry 270, 4264-4271.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-May-2010 JSW
      SynonymsAnti-TRPV2
      ApplicationFrozen Sections (1 µg/ml)
      Immunoblotting (2 µg/ml, see application references)
      Immunocytochemistry (2 µg/ml, see application references)
      Application Data
      Detection of mouse vanilloid-receptor-like protein 1 by staining frozen sections. Sample: Mouse spinal cord dorsal horn fixed in 4% paraformaldehyde. Primary antibody: Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb (Cat. No. PC421) (1 µg/ml). Staining was completely abolished by preincubating the purified antibody with control peptide at 10-6 M (data not shown).

      Detection of rat vanilloid-receptor-like protein 1 by immunoblotting. Sample: Whole tissue extract from rat spinal cord pre-incubated without peptide (lane 1) or with control peptide (25 µg/ml, lane 2). Primary antibody: Anti-Vanilloid Receptor Like Protein-1 (744-761) Rabbit pAb (Cat. No. PC421) (2 µg/ml). Detection: chemiluminescence.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~98 kDa vanilloid receptor like protein-1. Supplied with a lyophilized control peptide (100 µg size only).
      BackgroundThe detection of noxious stimuli (chemical, mechanical, or thermal), a process referred to as nociception, occurs predominantly at the peripheral terminals of primary afferent neurons. This information is ultimately transmitted to the central nervous system to evoke a perception of pain which initiates appropriate protective reflexes. Vanilloid-containing compounds which include capsaicin, the active ingredient in hot chilli peppers, selectively depolarize nociceptors to activate the "nociceptive" pathway. The receptor for capsaicin, vanilloid receptor 1 (VR1), has been identified as a nonselective cation channel that resembles members of the transient receptor potential (TRP) family of ion channels. The vanilloid receptor 1 protein functions both as a receptor for capsaicin and a transducter of noxious thermal stimuli. Immunocytochemical and mRNA localization studies suggest that VR1 protein is localized to small-diameter sensory neurons within the dorsal root ganglia and nerve terminals in the dorsal horn. A capsaicin-receptor homolog has recently been identified, designated the vanilloid-receptor-like protein 1 (VRL-1). VRL-1 is activated by high temperatures and is expressed in medium- to large-diameter neurons within sensory ganglia.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide (KNSASEEDHLPLQVLQSP) corresponding to amino acids 744-761 of rat vanilloid receptor like protein 1, conjugated to KLH
      IsotypeIgG
      Speciesmouse, rat
      Positive controlRat or mouse spinal cord extract
      FormLiquid
      Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide (immunogen) (100 µg size only).
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide (antibody only)
      CommentsA protein database search confirms that this peptide sequence is unique to the Vanilloid-Receptor-Like Protein 1. No cross-reactivity with the capsaicin receptor protein is expected. This signal is completely abolished in immunoblotting by preincubating the affinity purified antibody with peptide at 25 µg/ml. Unconjugated Vanilloid-Receptor-Like Protein 1 Control Peptide has been included for use as an internal control for antibody specificity. Immunohistochemical staining results correlate well with other independent reports. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Special InstructionsReconstitute the control peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Store lyophilized control peptide at -20°C. Following reconstitution of the control peptide, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesStrokes, A.L., et al., 2004. J. Exp. Med. 200, 137.
      Caterina, M.J., et al., 1999. Nature 398, 436.
      Gau, A., et al., 1999. Eur. J. Neurosci. 11, 946.
      Michael, G.J. and Priestley, J.V., 1999. J. Neurosci. 19, 1844.
      Caterina, M.J., et al., 1997. Nature 389, 816.
      Szallasi, A., 1994. Gen. Pharmacol. 25, 223.
      Beven, S., and Szolcsanyi, J., 1990. Trends Pharmacol. Sci. 11, 330.
      Fields, H.L., 1987. Pain (New York: McGraw-Hill), 1987.
      Citation
    • Math P. Cuajungco, et al. (2006) PACSINs bind to the TRPV4 cation channel: PACSIN 3 modulates the subcellular localization of TRPV4. Journal of Biological Chemistry in press,.
    • Ricarda Jahnel, et al. (2003) Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression. European Journal of Biochemistry 270, 4264-4271.
      		•
      Application referencesImmunoblotting and Immunocytochemistry Jahnel, R., et al. 2003. Eur. J. Biochem. 270, 4264.