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PC315 Anti-VEGF (Ab-4) Rabbit pAb

Anti-VEGF (Ab-4) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Vascular Endothelial Growth Factor, Anti-Vascular Permeability Factor, Anti-VPF

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PC315
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~34-50 kDa isoforms of VEGF. Cat. No. PC315 is recommended for immunohistochemical staining of rodent tissues. Recognizes VEGF in human tissues by immunoblotting but not by immunohistochemistry.
      Catalogue NumberPC315
      Brand Family Calbiochem®
      SynonymsAnti-Vascular Endothelial Growth Factor, Anti-Vascular Permeability Factor, Anti-VPF
      Application Data
      Detection of human VEGF by immunohistochemistry. Sample: Human VEGF fixed in formalin and paraffin-embedded. Primary antibody: Anti-VEGF (Ab-4) Rabbit pAb (Cat. No. PC315) (5 µg/ml). Detection: fluorescence.
      References
      ReferencesSoker, S., et al. 1998. Cell 92, 735.
      Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
      Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
      Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
      Brown, L., et al. 1992. Kidney International 42, 1457.
      Dvorak, H., et al. 1991. J. Exp. Med. 174, 1275.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.6.
      Positive controlRecombinant VEGF or blood vessels in skin or intestine (rodent only)
      Preservative15 mM sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Application NotesParaffin Sections (1:200-1:400, pressure cooker pre-treatment required, see comments)
      Application CommentsTested on formalin-fixed, paraffin embedded tissue. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human VEGF₁₆₅
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing intitial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC315 0

      Documentation

      Anti-VEGF (Ab-4) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-VEGF (Ab-4) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC315

      References

      Reference overview
      Soker, S., et al. 1998. Cell 92, 735.
      Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
      Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
      Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
      Brown, L., et al. 1992. Kidney International 42, 1457.
      Dvorak, H., et al. 1991. J. Exp. Med. 174, 1275.

      Brochure

      Title
      Angiogenesis and Tumor Metastasis Brochure and Technical Guide
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-March-2018 JSW
      SynonymsAnti-Vascular Endothelial Growth Factor, Anti-Vascular Permeability Factor, Anti-VPF
      ApplicationParaffin Sections (1:200-1:400, pressure cooker pre-treatment required, see comments)
      Application Data
      Detection of human VEGF by immunohistochemistry. Sample: Human VEGF fixed in formalin and paraffin-embedded. Primary antibody: Anti-VEGF (Ab-4) Rabbit pAb (Cat. No. PC315) (5 µg/ml). Detection: fluorescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~34-50 kDa isoforms of VEGF.
      BackgroundVascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is a secreted heparin binding cytokine expressed in tumor and animal cells. VEGF has been implicated in the control of angiogenesis due to its selective mitogenic stimulation of vascular endothelial cells and enhancement of vascular permeability. Other effects of VEGF on vascular endothelial cells include; increasing intracellular calcium, stimulating inositol triphosphate formation, induction of von Willebrand factor release, and stimulating tissue factor expression. VEGF is a ~34-50 kDa dimer of two identical disulfide-linked subunits existing in four molecular species; 121, 165, 189, and 206 amino acid residues long, arising from alternative splicing of a single gene. VEGF165 and VEGF121 are secreted proteins and act as diffusible agents, whereas VEGF189 and VEGF206 remain cell associated. VEGF binds to two receptor tyrosine kinases, Flt-1 (VEGFR1) and KDR/Flk-1 (VEGFR2). Recently, a third VEGF receptor was identified and cloned from tumor cells. This VEGF receptor is isoform-specific, binding VEGF165 but not VEGF121. Studies indicate that this VEGF receptor (VEGF165R) is identical to human neuropilin-1, a receptor for the collapsin/semaphorin family that mediates neuronal cell guidance.
      HostRabbit
      Immunogen speciesHuman
      Immunogenrecombinant, human VEGF₁₆₅
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlRecombinant VEGF or blood vessels in skin or intestine (rodent only)
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.6.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative15 mM sodium azide
      CommentsTested on formalin-fixed, paraffin embedded tissue. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing intitial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSoker, S., et al. 1998. Cell 92, 735.
      Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem. 272, 32521.
      Klagsbrun, M. and Soker, S. 1993. Current Biology 3, 699.
      Senger, D., et al. 1993. Cancer and Metastasis Rev. 12, 303.
      Brown, L., et al. 1992. Kidney International 42, 1457.
      Dvorak, H., et al. 1991. J. Exp. Med. 174, 1275.