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PC287 Anti-AML3 Rabbit pAb

Anti-AML3, rabbit polyclonal, recognizes the ~65, ~60, and ~45 kDa AML3 proteins in transfected COS or buffalo rat liver cells. It is validated for gel shift and Western blotting.

Anti-AML3 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC287
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~65 kDa, the ~60 kDa, and the ~45 kDa AML3 proteins in transfected COS or BRL (buffalo rat liver) cells.
      Catalogue NumberPC287
      Brand Family Calbiochem®
      References
      ReferencesBanerjee, C., et al. 1997. J. Cell Biochem. 66, 1.
      Lo Coco, F., et al. 1997. Haematologica 82, 364.
      Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
      Levanon, D., et al. 1994. Genomics 23, 425.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlTransfected COS or buffalo rat liver (BRL) cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesGel Shift Banerjee, C., et al. 1997. J. Cell Biochem. 66, 1. Lo Coco, F., et al. 1997. Haematologica 82, 364. Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251. Levanon, D., et al. 1994. Genomics 23, 425.
      Key Applications Gel Shift
      Immunoblotting (Western Blotting)
      Not Frozen Sections
      Not Immunofluorescence
      Not Immunoprecipitation
      Not Paraffin Sections
      Application NotesGel Shift (see application references)
      Immunoblotting (2.5 µg/ml)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      Application CommentsThe ~45 kDa species is not detected in mature osteoblasts. For gel shift assay resuspend in 100 µl of buffer. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide [(K)TDVPRRISDDDTATSD] corresponding to amino acids 319-334 from human AML3
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC287 0

      Documentation

      Anti-AML3 Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC287

      References

      Reference overview
      Banerjee, C., et al. 1997. J. Cell Biochem. 66, 1.
      Lo Coco, F., et al. 1997. Haematologica 82, 364.
      Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
      Levanon, D., et al. 1994. Genomics 23, 425.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-July-2009 RJH
      ApplicationGel Shift (see application references)
      Immunoblotting (2.5 µg/ml)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes proteins of ~65, ~60 and ~45 kDa in osteoclasts and bone.
      BackgroundThe family of nuclear transcription factors represented by AML are important transactivators of tissue-specific genes of hematopoietic and bone cell lineage. There are currently three mammalian proteins that are members of this family, AML1, AML2, and AML3. The AML proteins in turn form heterodimers with another protein, CBFβ and it is this complex that produces the transcription factor activity. Within this complex the AML protein provides the DNA binding function. The AML gene was discovered in 1991 by cloning the t(8;21) translocation that is common to AML. Structurally the AML protein contains a transactivation domain at the C-terminus is homologous with the Drosophila runt gene that acts as the DNA binding area of the protein. AML3, the third member of the AML family, is unique from the other family members in that it also possesses osteoblast-specific transcriptional regulation properties. AML3 has been demonstrated as a single transcript of 5.4 kb in bone tissues where the protein encoded by the gene has been shown to be present in the osteoblast-specific promoter binding complex. Like other AML proteins, AML3 contains the runt domain, a putative ATP binding site, and a proline and serine rich C-terminal half. AML3 is also the predominant runt domain containing transcription factor in Buffalo Rat liver cells.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide [(K)TDVPRRISDDDTATSD] corresponding to amino acids 319-334 from human AML3
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlTransfected COS or buffalo rat liver (BRL) cells
      FormLiquid
      FormulationIn PBS.
      PreservativeNone
      CommentsThe ~45 kDa species is not detected in mature osteoblasts. For gel shift assay resuspend in 100 µl of buffer. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°).
      Toxicity Standard Handling
      ReferencesBanerjee, C., et al. 1997. J. Cell Biochem. 66, 1.
      Lo Coco, F., et al. 1997. Haematologica 82, 364.
      Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
      Levanon, D., et al. 1994. Genomics 23, 425.
      Application referencesGel Shift Banerjee, C., et al. 1997. J. Cell Biochem. 66, 1. Lo Coco, F., et al. 1997. Haematologica 82, 364. Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251. Levanon, D., et al. 1994. Genomics 23, 425.