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OP115 Anti-MDM2 (Ab-2) Mouse mAb (2A10)

This Anti-MDM2 (Ab-2) Mouse mAb (2A10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-2).

Anti-MDM2 (Ab-2) Mouse mAb (2A10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Anti-Murine Double Minute Chromosome-2

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OP115
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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OP115-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells.
      Catalogue NumberOP115
      Brand Family Calbiochem®
      SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Anti-Murine Double Minute Chromosome-2
      Application Data
      Detection of human MDM2 by immunoblotting. Sample: Whole cell lysate from A549 cells. Primary antibody: Anti-MDM2 (Ab-2) Mouse mAb (2A10) (Cat. No. OP115) (2 μg/ml). Detection: chemiluminescence.
      References
      ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
      Barak, Y., et al. 1993. EMBO. J. 12, 461.
      Chen, J., et al. 1993. Mol. Cell Biol. 13, 4107.
      Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
      Leach, F.S., et al. 1993. Cancer Res. 53, 2231.
      Oliner, J.D., et al. 1993. Nature 362, 857.
      Momand, J., et al. 1992. Cell 69, 1237.
      Oliner, J.D., et al. 1992. Nature 358, 80.
      Fakharzadeh, S.S., et al. 1991. EMBO. J. 10, 1565.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlA549 cells or most tissues
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation, Original Clone Chen, J., et al. 1993. Mol. Cell Biol. 13, 4107.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (2 µg/ml)
      Immunofluorescence (1 µg/ml)
      Immunoprecipitation (1 µg/reaction, see application references)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenhuman MDM2 protein
      ImmunogenHuman
      Epitopewithin amino acids 294-339
      Clone2A10
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP115-100UG 04055977224955

      Documentation

      Anti-MDM2 (Ab-2) Mouse mAb (2A10) Certificates of Analysis

      TitleLot Number
      OP115

      References

      Reference overview
      Marchetti, A., et al. 1995. J. Pathol. 175, 31.
      Barak, Y., et al. 1993. EMBO. J. 12, 461.
      Chen, J., et al. 1993. Mol. Cell Biol. 13, 4107.
      Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
      Leach, F.S., et al. 1993. Cancer Res. 53, 2231.
      Oliner, J.D., et al. 1993. Nature 362, 857.
      Momand, J., et al. 1992. Cell 69, 1237.
      Oliner, J.D., et al. 1992. Nature 358, 80.
      Fakharzadeh, S.S., et al. 1991. EMBO. J. 10, 1565.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Anti-Murine Double Minute Chromosome-2
      ApplicationImmunoblotting (2 µg/ml)
      Immunofluorescence (1 µg/ml)
      Immunoprecipitation (1 µg/reaction, see application references)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
      Application Data
      Detection of human MDM2 by immunoblotting. Sample: Whole cell lysate from A549 cells. Primary antibody: Anti-MDM2 (Ab-2) Mouse mAb (2A10) (Cat. No. OP115) (2 μg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the ~90 kDa (apparent MW) MDM2 protein.
      BackgroundIncreased expression of proto-oncogenes due to gene amplification can lead to cellular transformation in the absence of other mutagenic events. Recently, a novel gene termed MDM2, first identified in mouse as a small acentromeric extrachromosomal element, was shown to become oncogenic upon amplification and tumorigenic when overexpressed in NIH3T3 and Rat2 cells. The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas. Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation. MDM2 contains two putative metal-binding motifs, one of which shares similarity to known zinc finger transcriptional activators. MDM2 also contains a putative nuclear localization signal and an acidic domain of the type found in transcriptional activators. A functional role for MDM2 has recently been identified on the basis of experiments which demonstrate that MDM2 forms a stable complex with p53 in vivo. Furthermore, over-expression of MDM2 inhibits the ability of wild type p53 to stimulate expression of target genes, and this inhibition appears to result from MDM2 binding to the acidic activation domain of p53, thereby preventing p53 from directly contacting the transcriptional machinery. Interestingly, wild type p53 appears to positively regulate expression of the MDM2 gene. MDM2 amplification has been observed in sarcomas, and direct analysis of the MDM2 and p53 genes in sarcomas indicates that one or the other but not both of these genes is mutated in 70% of the tumors. Thus, it appears that genetic alterations in either p53 or MDM2 represent alternative mechanisms for inactivating the same growth suppressive pathway. Regulation of MDM2 expression by p53 also represents a potential feed back control of p53 function.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman MDM2 protein
      Epitopewithin amino acids 294-339
      Clone2A10
      IsotypeIgG2a
      Specieshuman
      Positive controlA549 cells or most tissues
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
      Barak, Y., et al. 1993. EMBO. J. 12, 461.
      Chen, J., et al. 1993. Mol. Cell Biol. 13, 4107.
      Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
      Leach, F.S., et al. 1993. Cancer Res. 53, 2231.
      Oliner, J.D., et al. 1993. Nature 362, 857.
      Momand, J., et al. 1992. Cell 69, 1237.
      Oliner, J.D., et al. 1992. Nature 358, 80.
      Fakharzadeh, S.S., et al. 1991. EMBO. J. 10, 1565.
      Application referencesImmunoprecipitation, Original Clone Chen, J., et al. 1993. Mol. Cell Biol. 13, 4107.