Millipore Sigma Vibrant Logo

MAC140 Anti-Inntag™ 10 (IT10) Antibody, clone IT10

Anti-Inntag 10 (IT10), clone IT10 is a mouse monoclonal antibody that detects the Innocuous Inntag IT10 based on pollen allergen Abt t5 sequence and is tested for use in Immunocytochemistry and Western Blotting.

Anti-Inntag™ 10 (IT10) Antibody, clone IT10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Gene Symbol: N/A UniProt Number: P10414
View Products on Sigmaaldrich.com
MAC140
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAC140
      DescriptionAnti-Inntag™ 10 (IT10) Antibody, clone IT10
      Alternate Names
      • Allergen Amb t V
      • Allergen Ra5G
      • Amb t 5
      Background InformationPollen allergen Amb t 5 (UniProt: P10414; also known as Allergen Amb t V, Allergen Ra5G, Amb t 5) binds to IgE and causes allergic reaction in human. Inntag IT10 is a small protein domain of Ambrosia trifida (Giant ragweed) that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it does not compromise cell fitness when overexpressed, and has no detectable functional and localization effects when fused to a variety of target proteins. Structure of the Inntag IT10 is based on a packed beta-sheet conformation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Inntag 10 (IT10), clone IT10 is a mouse monoclonal antibody that detects the Innocuous Inntag IT10 based on pollen allergen Abt t5 sequence and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Inntag 10 (IT10) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

      Immunocytochemistry Analysis: A representative lot detected Inntag 10 (IT10) in Immunocytochemistry applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
      Biological Information
      ImmunogenHis-tagged recombinant peptide corresponding to 40 amino acids from Ambrosia trifida.
      CloneIT10-1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone IT10-1 detects pollen allergen Abt t 5 in HEK293 cells transfected with GFP-IT10.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteAmbrosia trifida.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N/A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~33.7 kDa observed; 8.06 kDa calculated. 33.7 kD observed corresponds to 29.4 kDa GFP plus 4.3 kDa IT10. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in transfected HEK293/GFP-IT10 cell lysates.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 10 (IT10) in 10 µg of transfected HEK293/GFP-IT10 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAC140 04054839240591