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MABT900 Anti-Connexin 45 Antibody, clone P3C9

This mouse monoclonal Anti-Connexin 45, clone P3C9, Cat. No. MABT900 is tested for use in Western Blotting for the detection of Connexin-45.

Anti-Connexin 45 Antibody, clone P3C9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Gjc1, Gja7 UniProt Number: A4GG66
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MABT900
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT900
      DescriptionAnti-Connexin 45 Antibody, clone P3C9
      Alternate Names
      • Gap junction gamma-1 protein
      • Cx45
      • Gap junction alpha-7 protein
      Background InformationGap junction gamma-1 protein (UniProt: A4GG66; also known as Connexin-45, Cx45, Gap junction alpha-7 protein) is encoded by the Gjc1 (also known as Gja7) gene (Gene ID: 266706) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-45 is a multi-pass membrane protein with ubiquitous expression. It consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weights diffuse from one cell to a neighboring cell. Connexin 45 is the first cardiac connexin expressed during embryonic development and plays an important role in propagating the action potential from the conduction system to the working myocardium. Connexin 45 gap junction channels are reported to close when the membrane potential becomes negative. This prevents retrograde conduction from myocardium to the conduction system.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-Connexin 45, clone P3C9, Cat. No. MABT900 is tested for use in Western Blotting for the detection of Connexin-45.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Connexin-45 in Western Blotting applications (Manias, J.L., et. al. (2008). Cardiovasc Res. 80(3):385-95).

      Western Blotting Analysis: A representative lot detected Connexin-45 in Western Blotting applications (Kopanic, J.L., et. al. (2013). Front Pharmacol. 4:106).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of rat connexin 45.
      CloneP3C9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone clone P3C9 detects Connexin-45 in rat and mouse cells. It targets an epitope within 14 amino acids from the C-terminal half of rat connexin 45.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteRat, Mouse. Predicted to react with Bovine, Canine, Chicken, Human, Porcine, Rabbit, and Rhesus Macaque based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gjc1
      • Gja7
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~45 kDa observed; 45.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse heart tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Connexin 45 in 10 µg of mouse heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT900 04054839198953